反相液相色谱法测定芦荟制品中芦荟苷的含量

食品科学 ›› 2005, Vol. 26 ›› Issue (8): 349-351.

反相液相色谱法测定芦荟制品中芦荟苷的含量

龙洲雄, 万春花, 胡海山, 王凯, 张云伟

江西省分析测试研究所

出版日期:2005-08-15 发布日期:2011-09-19

Determination of Aloin in Aloe Products by RP-HPLC

LONG Zhou-Xiong, WAN Chun-Hua, HU Hai-Shan, WANG Kai, ZHANG Yun-Wei

Jiangxi Institute of Analysing and Testing

Online:2005-08-15 Published:2011-09-19

摘要/Abstract

摘要： 采用甲醇直接超声提取、反相液相色谱法测定芦荟制品中芦荟苷的含量。使用KromasilC18柱,乙腈:水(体积比25:75)为流动相,流速1.0ml/min,波长359nm。方法平均回收率97.8%,RSD在0.75～3.8%(n=5)之间。本法简便、快速、准确可靠。

关键词: 反相液相色谱法, 芦荟制品, 芦荟苷

Abstract: This paper reported a method for determining aloin in aloe products by RP-HPLC. After being extracted with methanolby ultrasonic, aloin was separated on a Kromasil C18 column with acetonitrile-water(25:75 by volume) as a mobile phase at a flowrate of 1.0ml/min and detected at 359nm. The average recovery was 97.8% and the relative standard deviations(n=5) was 0.75～3.8%. This method was proved to be simple,rapid,accurate and reliable.

Key words: RP-HPLC, aloe product, Aloin

龙洲雄, 万春花, 胡海山, 王凯, 张云伟. 反相液相色谱法测定芦荟制品中芦荟苷的含量[J]. 食品科学, 2005, 26(8): 349-351.

LONG Zhou-Xiong, WAN Chun-Hua, HU Hai-Shan, WANG Kai, ZHANG Yun-Wei. Determination of Aloin in Aloe Products by RP-HPLC[J]. FOOD SCIENCE, 2005, 26(8): 349-351.

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