食品科学 ›› 2005, Vol. 26 ›› Issue (8): 404-407.

• 营养卫生 • 上一篇    下一篇

葛仙米藻红蛋白体外抗活性氧自由基作用的研究

 汪兴平, 谢笔均, 潘思轶, 陈德文   

  1. 华中农业大学食品科技学院,湖北民族学院生物科学与技术学院
  • 出版日期:2005-08-15 发布日期:2011-09-19

The Studies on in vitro Anti-oxygen Free Radical of Nostoc Sphaeroids kutz Phycoerythrin

 WANG  Xing-Ping, XIE  Bi-Jun, PAN  Si-Yi, CHEN  De-Wen   

  1. 1.College of Food Science and Technology, Huazhong Agriculture University; 2.School of Biological Science and Technology, Hubei Institute for Nationalities
  • Online:2005-08-15 Published:2011-09-19

摘要: 葛仙米以其资源稀少、营养价值丰富,独特的生物活性和药理作用而引起人们广泛地关注。为探讨葛仙米中的生物活性成分,本文以野生葛仙米藻红蛋白为研究对象,在系统研究葛仙米藻红蛋白的提取、分离和纯化的基础上,采用微弱化学发光法,研究了葛仙米藻红蛋白清除氧自由基的能力。结果表明:清除氧自由基的能力为:粗提物>藻红蛋白>藻蓝蛋白;葛仙米藻红蛋白清除氧自由基的能力为:H2O2自由基的清除率>•OH自由基的清除率>O2•自由基的清除率。

关键词: 葛仙米, 藻红蛋白, 体外, 抗氧自由基

Abstract: The significant biological and pharmacological activity of Nostoc Sphaeroids kutz with abound nutrition and infrequent distribution had been paid people’s much attention. In order to ascertain its active component, the phycoerythin was used as material. On the basis of systemically studies on preparation and purification of phycoerythrin, Scavenge capability of oxygen free radical was studied by chemiluminescence. The main results were shown that scavenge capability of oxygen free radical was orderly crud extract, phycoerythrin, phycocynin, and PE was orderly, H 2 O 2 radical,• OH radical, O 2 •radical, respectively.

Key words: Nostoc Sphaeroids kutz, phycoerythrin, in vitro, antioxygen free radical activity