食品科学 ›› 2006, Vol. 27 ›› Issue (1): 90-92.

• 基础研究 • 上一篇    下一篇

不同培养条件对冬虫夏草菌丝体甘露醇的影响

 刘彦威, 苏敬良, 韩博, 田向荣   

  1. 河北工程学院农学院; 中国农业大学兽医学院
  • 出版日期:2006-01-15 发布日期:2011-09-05

Effects on Determining Mannitol Content of Myceliun of Cordyceps Sinensis byD ifferentC ultureC ondition

 LIU  Yan-Wei, SU  Jing-Liang, HAN  Bo, TIAN  Xiang-Rong   

  1. 1.Hebei Engineering College; 2.Animal Medicine College, China Agricultural University.
  • Online:2006-01-15 Published:2011-09-05

摘要: 目的:探讨不同培养条件对冬虫夏草菌丝重量和甘露醇含量的影响。方法:甘露醇的测定采用比色方法,菌丝干燥后直接称重。结果对于菌丝的产量,摇菌时间不应超过16d,最佳氮源为硝酸铵。对培养液和菌丝中甘露醇的总量,摇菌时间不应超过8d,最佳氮源为氯化铵。结论:氮源和摇菌时间不同,对冬虫夏草菌的菌丝重量和甘露醇含量有影响。

关键词: 培养条件, 冬虫夏草菌丝, 甘露醇

Abstract:  Object: To study the effects of mannitol content and mycelium weight by different culture condition in mycelium of cordyceps sinensis. Method: Colorimetric method was used to determine mannitol content. Mycelium weight was weighted after drying. Result: The mycelium weight was maximum at 16 days of shaking with ammonium nitrate as the optimum source of nitrogen. To obtain a high mannitol content in culture and mycelium, it was necessary to have 8 days of shaking and the optimum source of nitrogen was ammonium ohloride. Conclusion: The mannitol content and mycelium weight were affected by shaking time and source of nitrogen.

Key words: culture condition, mycelium, mannitol