大孔树脂对薯蔓黄酮吸附分离特性研究

食品科学 ›› 2006, Vol. 27 ›› Issue (10): 423-427.

大孔树脂对薯蔓黄酮吸附分离特性研究

洪雪娥, 高荫榆, 罗丽萍, 夏冬华, 陈才水

南昌大学食品科学教育部重点实验室

出版日期:2006-10-15 发布日期:2011-11-16

Study on Adsorption and Separation of Flavonoids from Sweet Potato Vines with Macroporous Resin

HONG Xue-E, GAO Yin-Yu, Luo-Li-Ping, XIA Dong-Hua, Chen-Cai-Shui

Key Laboratory of Food Science, Ministry of Education, Nanchang University, Nanchang 330047, China

Online:2006-10-15 Published:2011-11-16

摘要/Abstract

摘要： 本文研究了大孔树脂对薯蔓黄酮(FSPV)吸附分离特性。选择8种大孔吸附树脂,比较其对FSPV的吸附率和解吸率,筛选出最佳种类,并对其动力学曲线和动态吸附性能进行了考察。结果表明:AB-8树脂对FSPV有较好的吸附和解吸效果;其吸附FSPV适宜的条件为:上样液浓度06～1.0mg/ml,pH值在4.28左右,上样流速3ml/min;用95%乙醇洗脱时,解吸率达96.78%,4BV洗脱液基本上能将FSPV洗脱下来。AB-8树脂综合性能较好,适合于FSPV的分离纯化。

关键词: 薯蔓, 黄酮, 大孔树脂, 吸附分离

Abstract: To study the absorption and separation of the macroporous resin for flavonoids from sweet potato vines (FSPV). Eight types of macroporous resin were selected to compare their performances in absorbing and desorbing FSPV. The optimal type for FSPV was decided, meanwhile, its kinetic curve and dynamic absorbing behavior were studied. Experimental resultsshowed that AB-8 resin possesses higher absorption and desorption capacity. The appropriate adsorption conditions were: the range of the sample concentration was 0.6～1.0mg/ml, pH was 4.28 or so and velocity of flow was 3ml/min .When 95% ethanol was used as eluant, the desorption capacity was 97%, and 4 BV eluant could desorb FSPV basically. AB-8 macroporous resin showed better comprehensive adsorption property. It can be used to purify and separate FSPV.

Key words: sweet potato vines, flavonoid, macroporous resin, adsorption and separation

洪雪娥, 高荫榆, 罗丽萍, 夏冬华, 陈才水. 大孔树脂对薯蔓黄酮吸附分离特性研究[J]. 食品科学, 2006, 27(10): 423-427.

HONG Xue-E, GAO Yin-Yu, Luo-Li-Ping, XIA Dong-Hua, Chen-Cai-Shui. Study on Adsorption and Separation of Flavonoids from Sweet Potato Vines with Macroporous Resin[J]. FOOD SCIENCE, 2006, 27(10): 423-427.

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