关键词: 转基因产品, 外源基因, 克隆, 转化

Abstract:  The primers with different enzyme sites were designed, and the plant endogenous rbcL gene and two kinds of universal exogenous CP4-EPSPS gene and BAR gene in genetically modified products were expanded by PCR respectively. The PCR products were ligated with pGEM-T Easy Vector, then cloned into E.coli strain DH5α. The clone plasmids of the 3 genes were extracted, then analysed with restriction enzyme and sequenced respectively. The enzymed products of the 3 clone plasmids cut by two corresponding restriction enzyme were recovered, and transformed into the received carrier pCAMBW one after the other. The restriction enzyme analysising and PCR detection results of the recombinant plasmid obtained finally showed that the 3 genes were transformed successfully into the received carrier.

Key words:  , genetically modified product； exogenous gene； clone； transform；