食品科学 ›› 2006, Vol. 27 ›› Issue (2): 192-195.

• 工艺技术 • 上一篇    下一篇

超滤在大豆多肽分离纯化中应用

  邓成萍, 薛文通, 孙晓琳, 全明海   

  1. 中国农业大学食品科学与营养工程学院; 保定味群食品工业有限公司
  • 出版日期:2006-02-15 发布日期:2011-09-05

Application of Ultrafiltration on Separation and Purification of Soybean Peptides

   Deng-Cheng-Ping, XUE  Wen-Tong, SUN  Xiao-Lin, QUAN  Ming-Hai   

  1. 1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2.Baoding Way Chein Food Industrial Co. Ltd., Baoding 071000, China
  • Online:2006-02-15 Published:2011-09-05

摘要: 本试验应用超滤技术对大豆多肽进行分离,研究了超滤系统几个主要参数对膜通量的影响。研究结果表明:截留分子量30000Da的超滤膜应在压力0.12MPa、温度40℃、pH值7.0下运行40min为一个周期,膜通量为19L/m2·h左右;截留分子量10000Da的超滤膜应在压力0.10MPa、温度<45℃、自然pH值下运行60min为一个周期,膜通量为25L/m2·h左右;截留分子量5000Da的超滤膜应在压力0.10MPa、温度<45℃、自然pH值下运行80min为一个周期,膜通量为22L/m2·h左右;经分离得到分子量>30000Da的大豆多肽约占13.21%,分子量10000~30000Da的大豆多肽约占4.05%,分子量5000~10000Da的大豆多肽约占6.41%,分子量<5000Da的大豆多肽约占76.11%。

关键词: 大豆多肽, 超滤, 分离, 纯化

Abstract: In this experiment, soybean peptides were separated by ultrafiltration. How those major ultrafiltration-system parameters affecting the membrane flux were studied. It has showed that the ultrafiltration membrane with 30000 MWCO (Molecular Weight Cutoff) should run 40min for a cycle at 0.12MPa, 40℃ and pH7.0 and under this condition, the membrane flux would be about 19L/m·2h; when the ultrafiltration membrane with 10000 MWCO should run 60min for a cycle at 0.10MPa, <45℃ and natural pH and under this condition, the membrane flux would be about 25L/m2·h; when the ultrafiltration membrane with 5000 MWCO should run 80min for a cycle, at 0.10MPa, <45℃ and natural pH and under this condition, the membrane flux would be about 22L/m2·h. In the end, soybean peptides were separated according to its molecular weight: 13.21% with molecular weight >30000Da; 4.05% with molecular weight 10000~30000Da; 6.41% with molecular weight 5000~10000Da; and 76.11% with molecular weight <5000Da.

Key words: soybean peptides, ultrafiltration, separation, purification