啤酒酵母β-D-葡聚糖吸附毒素玉米赤霉烯酮(ZEA)的研究

食品科学 ›› 2006, Vol. 27 ›› Issue (4): 75-78.

啤酒酵母β-D-葡聚糖吸附毒素玉米赤霉烯酮(ZEA)的研究

张丽霞, 徐学明

江南大学食品学院

出版日期:2006-04-15 发布日期:2011-09-13

Adsorptive Properties of β-D-Glucans Extracted from Spent Brewer Yeast Cell Wall against Zearalenone

ZHANG Li-Xia, XU Xue-Ming

College of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China

Online:2006-04-15 Published:2011-09-13

摘要/Abstract

摘要： 本文用碱法,碱酸法和酶碱法从啤酒废酵母中提取碱不溶性葡聚糖,对产品进行了定性定量分析,得到的产品主要为β-D-葡聚糖,且不含甘露糖。研究了三种产品对真菌毒素玉米赤霉烯酮(ZEA)的吸附作用,实验结果表明,酶碱法提取的葡聚糖具有较好的吸附效果,在反应条件:β-D-葡聚糖100μg/ml,玉米赤霉烯酮40μg/ml,37℃,200r/min振荡2h,吸附量最大可达到2.296μg/mg葡聚糖。

关键词: 玉米赤霉烯酮；真菌毒素；啤酒酵母； &beta, -D-葡聚糖；吸附；

Abstract: β-D-glucans were extracted from the cell wall of spent brewer’s yeast (Saccharomyces cerevisiae) by alkali, acid -alkali or enzyme-alkali method, respectively. The basic components of the three different extracted products were analyzed. Paper chromatography and IR spectrum analysis showed that D-mannose was not found in all of them. The adsorptive properties of β-D-glucans on Zearalenone(ZEA) were determined by HPLC. It indicated that the absorptive capacity was different to β- D-glucans with different extracting methods. β-D-glucans obtained by enzyme-alkali method showed a relatively high adsorp- tion capacity for ZEA with a maximum adsorption of up to 2.296μg ZEA/mg glucan.

Key words: zearalenone； mycotoxin； brewer yeast； &beta, -D-glucan； adsorption；

张丽霞, 徐学明. 啤酒酵母β-D-葡聚糖吸附毒素玉米赤霉烯酮(ZEA)的研究[J]. 食品科学, 2006, 27(4): 75-78.

ZHANG Li-Xia, XU Xue-Ming. Adsorptive Properties of β-D-Glucans Extracted from Spent Brewer Yeast Cell Wall against Zearalenone[J]. FOOD SCIENCE, 2006, 27(4): 75-78.