食品科学 ›› 2006, Vol. 27 ›› Issue (6): 104-108.

• 基础研究 • 上一篇    下一篇

枯草芽孢杆菌fmbR抗菌物质稳定性研究

 别小妹, 吕凤霞,  陆兆新,  王冬立   

  1. 南京农业大学食品科技学院
  • 出版日期:2006-06-15 发布日期:2011-09-22

Study on Stability of Antimicrobial Substance Produced by Bacillus subtilis fmbR

 BIE  Xiao-Mei, 吕Feng-Xia ,   Lu-Zhao-Xin,   Wang-Dong-Li   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2006-06-15 Published:2011-09-22

摘要: 枯草芽孢杆菌fmbR能够产生抗菌物质,对革兰氏阳性菌和革兰氏阴性菌具显著的抑菌效果。本文研究了该抗菌物质对温度、pH和三种蛋白酶(胰蛋白酶、胰凝乳蛋白酶、胃蛋白酶)的稳定性。研究结果表明:抗菌物质在pH7.0下,在20~120℃的范围内,抑菌活性不发生变化;此外在室温下,pH处理在2.0~12.0范围内抗菌物质保持稳定,抑菌活性不受影响。结果还表明:抗菌物质对胃蛋白酶的作用表现出很高的稳定性,而胰蛋白酶和胰凝乳蛋白酶能够在一定条件下将抗菌物质降解。

关键词: 枯草芽孢杆菌fmbR, 抗菌物质, 温度, pH, 蛋白酶, 稳定性

Abstract: Bacillus subtilis fmbR is found to produce antimicrobial substance, which has exhibited significant inhibitory effect on Gram-positive bacteria and Gram-negative bacteria. In the paper, the stability of the antimicrobial substance on temperature, pH and three proteinases (trypsin, chymotrypsin and pepsin) was studied. The results showed that the antimicrobial substance is very stable within the tested range from 20℃ to 120℃ under pH7.0. In addition, the antimicrobial substance show no change from pH2.0~12.0 under 20℃. With regard to the three proteinases, the results indicated that the antimicrobial substance is sensitive to trypsin and chymotrypsin, but it is very stable to pepsin.

Key words:  , Bacillus subtilis fmbR; the antimicrobial substance; temperature; pH; proteinases; stability ;