食品科学 ›› 2007, Vol. 28 ›› Issue (1): 167-170.

• 生物工程 • 上一篇    下一篇

山茱萸中α-葡萄糖苷酶抑制活性因子的筛选(Ⅰ)

 马庆一, 陈丽华, 杨海延, 王庆玲   

  1. 郑州轻工业学院食品与生物工程学院; 郑州轻工业学院食品与生物工程学院 河南郑州450002; 河南郑州450002平顶山学院化学与化工学院; 河南平顶山467002; 河南郑州450002;
  • 出版日期:2007-01-15 发布日期:2011-12-31

Screeningα-Glucosidase Inhibitors from Coruns officinalis(I)

 MA  Qing-Yi, CHEN  Li-Hua, YANG  Hai-Yan, WANG  Qing-Ling   

  1. 1.Zhengzhou Institute of Light Industry, College of Food and Biological Engineering, Zhengzhou 450002, China; 2.College of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467002, China
  • Online:2007-01-15 Published:2011-12-31

摘要: 从山茱萸中提取、分离并部分纯化得到水粗提物以及多糖、鞣质、皂甙、有机酸等组分。它们的得率分别为9.60%、2.00%、2.40%、0.75%、4.10%。酶反应动力学检测结果表明,除有机酸外各组分均对α-葡萄糖苷酶有抑制作用,其中皂甙和鞣质效果较佳,优于两倍量拜堂平。实验证实了山茱萸是天然α-葡萄糖苷酶抑制剂的良好来源,而山茱萸鞣质和皂甙是降糖功能因子的良好候选物。

关键词: 山茱萸, &alpha, -葡萄糖苷酶抑制剂, 皂甙, 鞣质, 血糖

Abstract: In this experiment the crude eq-extracts and the components of polysaccharides, tannins, saponins and organic acids were obtained from Cornus officinalis by extraction, isolation and partial purification with yields respectively 9.60%, 2.00%, 2.40%, 0.75%, 4.10%. The results of enzymatic kinetics assays showed that all of the components have inhibitory activities against α-glucosidase except organic acids. Saponins and tannins showed better inhibition effect than double concentration of Glucobay. The experiment confirmed that Cornus officinalis is a good source of α-glucosidase inhibitors and Cornus officinaliss saponins and tannins may become promising candidates for blood sugar reducing function factors.

Key words:  , Cornus officinalis; &alpha, -glucosidase inhibitor; saponin; tannin; blood sugar;