食品科学 ›› 2007, Vol. 28 ›› Issue (5): 245-248.

• 生物工程 • 上一篇    下一篇

一株产白藜芦醇真菌的培养及调控研究

 曹庸, 唐永红, 卢成英, 黄早成   

  1. 华南农业大学食品学院; 湖南省林产化工工程重点实验室; 湖南省林产化工工程重点实验室 广东广州510642湖南省林产化工工程重点实验室; 湖南张家界427000; 广东广州510642吉首大学生物资源与环境科学学院; 湖南吉首416000;
  • 出版日期:2007-05-15 发布日期:2011-12-31

Study on Isolation of Resveratrol-producing Fungus and Its Accumulating Characteristics

 CAO  Yong, TANG  Yong-Hong, LU  Cheng-Ying, HUANG  Zao-Cheng   

  1. 1.College of Food Science,South China Agricultural University,Guangzhou 510642,China; 2.Key Laboratory for Forest Products and Chemical Industry Engineering of Hunan,Zhangjiajie 427000,China; 3.College of Biological Resources and Environmental Sciences,Jishou University,Jishou 416000,China
  • Online:2007-05-15 Published:2011-12-31

摘要: 在虎杖组织培养过程中分离出1株真菌,对其发酵液提取物进行TLC和HPLC分析证明能产生白藜芦醇,并对其培养条件及白藜芦醇积累特性进行了初步研究,通过正交优化试验得到最佳培养条件为:20g/L蔗糖、2g/L硝酸铵、自然pH值(6.8~7.0)、温度为28℃,转速100r/min。3mmol/L苯丙氨酸前体物、Mg2+、Zn2+能促进真菌B-39的生长及白藜芦醇的积累,其白藜芦醇含量能达8.6617μg/100ml。

关键词: 白藜芦醇, 真菌, 分离, 培养条件, 积累特征

Abstract: A fungus was separated from the tissue cultural processes of Polygonum Cuspidatum and the zymotic extracts were analyzed by TLC and HPLC.The fungus obtained is B-39 which can produce resveratrol.The optimized cultural conditions are: saccharine 20 g/L,NH4NO4 2 g/L,pH 6.8~7.0 and 100 r/min by orthogonal tests.3mmol/L Phe with Mg2+and Zn2+can increase the growth of the fungus B-39 and the content of resvertrol.The actual content resveratrol in the fungus is determined as 8.6617μg/100 ml by HPLC.

Key words: resveratrol, fungi, isolation, cultural conditions, accumulating characteristics