食品科学 ›› 2008, Vol. 29 ›› Issue (1): 55-59.

• 基础研究 • 上一篇    下一篇

人参与胡萝卜愈伤组织共培养的研究

杨晶, 张美萍, 王义,   

  1. 吉林农业大学生命科学学院; 吉林农业大学生命科学学院 吉林长春130118; 吉林长春130118;
  • 出版日期:2008-01-15 发布日期:2011-07-28

Study on Callus Co-culture of Panax ginseng and Daucus carota

 YANG  Jing,   Zhang-Mei-Ping,   Wang-Yi   

  1. College of Life Science, Jilin Agricultural University, Changchun 130118, China
  • Online:2008-01-15 Published:2011-07-28

摘要: 本研究采用正交试验设计,对人参(Panax ginseng)和胡萝卜(Daucus carota)两种植物组织共培养的培养基和培养条件进行了优化筛选,同时比较了人参愈伤组织与共培养物的皂苷含量。结果表明,人参与胡萝卜共培养所获得的愈伤组织在23℃,1000lx的条件下生长良好,最佳基质MS+2,4-D3mg/L+IBA2mg/L+KT0.5mg/L,在培养25d时皂苷含量达到最高,浓度达到1.6mg/L。共培养物的皂苷含量比人参愈伤组织皂苷含量高出6.25%,为人参与胡萝卜原生质体融合、获得营养型蔬菜奠定了基础。

关键词:  , 人参, 胡萝卜, 共培养, 人参皂苷

Abstract: With orthogonal test design, to optimize bolting of medium and culture conditions with co-culture of Panax ginseng and Daucus carota, the saponin contents of Panax ginseng and co-culture were simultaneously compared. The results showed that the callus of the co-culture of Panax ginseng and Daucus carota grow well on the condition of 23 ℃ and 1000 lx with the optimal medium MS+2,4-D 3 mg/L+IBA 2 mg/L+KT 0.5 mg/L. After 25 d culture, the saponin content of co-culture is 6.25% higher than that of Panax ginseng callus. It settles foundation for protoplast fusion of Panax ginseng and Daucus carota as to obtain trophic vegetables.

Key words: Panax ginseng, Daucus carota, co-culture, Panax saponin