食品科学 ›› 2008, Vol. 29 ›› Issue (10): 47-50.

• 基础研究 • 上一篇    下一篇

艾蒿黄酮体外抗氧化活性及对DNA氧化损伤的保护研究

 吴娜, 孙智达   

  1. 华中农业大学食品科技学院;
  • 出版日期:2008-10-15 发布日期:2011-12-08

Study on Antioxidant Activity in vitro and Protection Against DNA Oxidative Damage of Flavonoids of Artemisia argyi

 WU  Na, SUN  Zhi-Da   

  1. College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Online:2008-10-15 Published:2011-12-08

摘要: 采用四种化学发光体系研究艾蒿黄酮(flavonoids of Artemisia argyi Lé vl.et Vant,简称FAA)的体外抗氧化活性,及对DNA氧化损伤的保护作用。结果表明,艾蒿黄酮能有效地清除O2-·、·OH、H2O2、减轻或消除·OH对DNA的氧化损伤。艾蒿黄酮50%抑制浓度(IC50)分别为151.17、15.34、0.23、15.46mg/L。实验同时还比较了艾蒿黄酮和VC的抗氧化作用,结果表明,艾蒿黄酮抗氧化效应远远高于VC的。艾蒿黄酮是有效的、多功能的天然抗氧化剂和自由基清除剂,是值得进一步开发利用的天然产物。

关键词: 艾蒿黄酮, 体外抗氧化, 化学发光, 保护DNA受损伤

Abstract: Flavonoids of Artemisia argyi (FAA) were investigated for their radical scavenging activity in vitro by using four chemiluminescence systems. The results showed that FAA can effectively scavenge O2-·,·OH and H2O2,and reduce or prevent DNA oxidative damage caused by ·OH. The 50% inhibition concentrations(IC50) are 151.17,15.34,0.23 and 15.46 mg/L. Compared with VC,FAA have higher antioxidant activity are effective and multifunctional natural antioxidants and radical scavengers,and are worth further exploiting and utilizing.

Key words: flavonoids of Artemisia argy, iantioxidant activity in vitro, chemiluminescence methods, protection effect on DNA damage