选择不同提取剂制备斑点叉尾鮰鱼皮胶原蛋白的研究

食品科学 ›› 2008, Vol. 29 ›› Issue (9): 321-325.

选择不同提取剂制备斑点叉尾鮰鱼皮胶原蛋白的研究

吴缇, 陈舜胜

上海海洋大学食品学院；

出版日期:2008-09-15 发布日期:2011-12-08

Study on Different Extraction Methods of Collagen from Channel Catfish Skin with Different Solutions

WU Ti, CHEN Shun-Sheng

College of Food Science,Shanghai Ocean University,Shanghai 200090,China

Online:2008-09-15 Published:2011-12-08

摘要/Abstract

摘要： 选择不同提取剂对斑点叉尾鮰鱼皮胶原蛋白进行制备,并对胶原蛋白进行了部分定性研究。实验结果表明,4℃条件下,鮰鱼鱼皮先用0.1mol/LNaOH浸泡6h再用2.5%NaCl浸泡6h,比单独或同时使用NaOH、NaCl能更好的去除杂蛋白,用10%的异丙醇溶液去除脂肪,用0.1mol/L柠檬酸浸提3d能较好的提取胶原蛋白,无色无味,提取率可达34.67%。获得的胶原蛋白中甘氨酸含量丰富,质量分数为21.19%,其变性温度为50.2℃。SDS-PAGE实验结果表明,提取的鮰鱼皮酸溶胶原具有较高的纯度,含有两条α链,β链含量较高。鱼皮胶原蛋白溶液具有较高的黏度,且随着蛋白浓度的增大而增大。

关键词: , 斑点叉尾鮰；鱼皮；胶原蛋白；提取剂；氨基酸；黏度；变性温度；

Abstract: In this study, different reagents were chosen to extract collagen from channel catfish skin and partial characteristics of the collagen were ivestigated. The results showed that under the condition of 4 ℃, soaking the fish skins with 0.1mol/L NaOH for 6 hfollowed by with 2.5% NaCl for 6 h, can remove non-collagens better than soaking with NaOH and NaCl alone or commonly.Then the fish skins are soaked with 10% butyl alcohol to remove lipids. Finally the skins are extracted to obtain collagen with 0.1 mol/L citric acid for 3 days. The extraction rate of collagen is 34.67% and the collagen is colorless and tasteless. The collagen contains more than 21.19% glycine, and its denaturation temperature is 50.2 ℃. SDS-PAGE showed that the collagen is composed of two a chains, and the content of b chain is higher. The collagen has high viscosity, which increases as the concentration of the collagen rising .

Key words: channel catfish, skin, collagen, extraction reagent, amino acid, viscosity, denaturation temperature

吴缇, 陈舜胜. 选择不同提取剂制备斑点叉尾鮰鱼皮胶原蛋白的研究[J]. 食品科学, 2008, 29(9): 321-325.

WU Ti, CHEN Shun-Sheng. Study on Different Extraction Methods of Collagen from Channel Catfish Skin with Different Solutions[J]. FOOD SCIENCE, 2008, 29(9): 321-325.

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