关键词: 黑木耳, 富硒, 深层发酵, 胞外多糖

Abstract:

In order to gain selenium-rich Auricularia auricular, and improve its output and ameliorate its quality, this study investigated the optimum conditions for submerged fermentation of Auricularia auricular with sodium selenite as selenium source. The results showed that the optimum carbon source, nitrogen source, pH and fermentation temperature are glucose, bean cake powder, 5.3 and 28 ℃, respectively. Under these conditions, different concentrations of sodium selenite solution were added in the fermentation, and then the biomass of Auricularia auricular, output of crude polysaccharide and absorption rate of selenite were determined. It was indicated that the output of Auricularia auricula mycelium may be promoted when the concentration of sodium selenite solution is below 60 mg/kg, while the output is reduced when the concentration of sodium selenite solution is between 60 mg/kg and 100 mg/kg. Moreover, when the concentration of sodium selenite solution is 60 mg/kg, the absorption rate of selenium is the highest.

Key words: Auricularia auricular, selenium enrichment, submerged fermentation, extracellular polysaccharide