食品科学 ›› 2013, Vol. 34 ›› Issue (1): 131-134.

• 基础研究 • 上一篇    下一篇

蝉花虫草中核苷类成分的分离纯化和鉴定

陈安徽,陈宏伟,徐 洋,卓 涛,邵 颖*   

  1. 徐州工程学院食品生物工程学院
  • 收稿日期:2012-06-30 修回日期:2012-12-17 出版日期:2013-01-15 发布日期:2013-01-07
  • 通讯作者: 邵颖 E-mail:shyzhbo2005@126.com
  • 基金资助:

    江苏省科技计划项目

Purification and Identification of Nucleoside Components in Cordyceps cicadae Fruit Bodies

  • Received:2012-06-30 Revised:2012-12-17 Online:2013-01-15 Published:2013-01-07

摘要: 对蝉花虫草子实体中的核苷类成分进行分离纯化,并对获得的组分进行鉴定。结果显示:采用65%乙醇超声波法提取的蝉花虫草子实体提取物于10000r/min离心5min后,经反相HPLC制备液相制备(色谱条件为:流动相为0~100%甲醇,梯度洗脱,洗脱速率为15mL/min,进样量1mL,检测波长260nm),分离得到6种主要化合物,经鉴定,6种化合物均为核苷类产品,分别为胞嘧啶、尿苷、肌苷、鸟苷、腺苷和虫草素。对6种化合物进行纯度验证,结果表明,6种化合物的纯度均在95%以上,说明采用反相HPLC分离制备蝉花虫草中的核苷类成分方法是可行的,并且该方法简便、准确。

关键词: 蝉花, 核苷, 分离纯化, 鉴定

Abstract: The aim of this study was to extract, purify and identify nucleoside components of Cordyceps cicadae fruit bodies. Samples were extracted with 65% ethanol by ultrasonic-assisted extraction, and a supernatant was obtained from the resulting extract by centrifugation at 10000 r/min for 5 min and separated by RP-HPLC using 0—100% methanol as a mobile phase through gradient elution at a flow rate of 15 mL/min (1 mL injection volume, 260 nm detection wavelength). As a result, 6 final products were obtained and identified as cytosine, uridine, inosine,guanosine, adenosine and cordycepin, respectively, with a purify exceeding 95%. This study demonstrates that RP-HPLC is a feasible, simple and accurate technique for separating and preparing nucleoside components of Cordyceps cicadae fruit bodies.

Key words: Cordyceps cicadae, nucleoside, purification, identification

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