携带SXT/R391家族整合接合元件多重耐药菌株的高效筛选与分析

doi:10.7506/spkx1002-6630-201513016

食品科学

携带SXT/R391家族整合接合元件多重耐药菌株的高效筛选与分析

孙凤娇，贺羽，陈兰明*

农业部水产品贮藏保鲜质量安全风险评估重点实验室（上海），上海海洋大学食品科学与技术学院，上海 201306

出版日期:2015-07-15 发布日期:2015-07-08

Effective Screening for Multiple-Drug Resistant Bacteria Harboring SXT/R391 Family of Integrative and Conjugative Elements

SUN Fengjiao, HE Yu, CHEN Lanming*

Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of
Agriculture, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

Online:2015-07-15 Published:2015-07-08

摘要/Abstract

摘要：

本研究建立了一种抗生素平板与分子检测相结合的高效筛选水产品中携带SXT/R391家族整合接合元件（integrative and conjugative elements，ICEs）的多重耐药菌株的方法。采用美国临床与实验室标准研究所标准Kirby-Bauer纸片扩散法、需氧菌液体微量稀释法，以及接合实验，对筛选、鉴定获得的携带SXT/R391家族ICEs的变形杆菌（Proteus vulgaris）进行了抗菌素、重金属抗性以及ICEs元件接合转移活性的分析。结果表明：运用本研究建立的筛选法可有效获得含有多重耐药基因的ICEs元件，其检出率为3%，远高于常规聚合酶链式反应技术的检测水平（＜1‰）。受试菌株对六大类10 种抗菌素均表现出抗性，并且对重金属镉、铜、锌和汞具有高度耐受性。接合实验证明了受试菌株所携带的ICEs元件在普通变形杆菌与大肠杆菌（Escherichia coli）MG1655之间的自我转移活性。

关键词: 整合接合元件, SXT/R391家族, 多重耐药, 重金属抗性

Abstract:

In this study, we established a highly effective method to screen multiple-drug resistant (MDR) bacteria carrying
the integrative and conjugative elements (ICEs). Five Proteus vulgaris strains harboring SXT/R391 family of ICEs, isolated
from aquatic products in Shanghai, China, were obtained using the new method. Antimicrobial activity and heavy metal
susceptibility of the P. vulgaris strains were examined using standard Kirby-Bauer disk diffusion method and dilution
susceptibility tests according to the Clinical and Laboratory Standards Institute (CLSI, USA, 2012 Edition), all showing
distinct resistance to ten antimicrobial agents belonging to six drug classes tested. In addition, a wide heavy metal resistance
profile was also detected, displaying strong resistance to Cd, Cu, Zn and Hg. The conjugation experiments demonstrated the
capacities of active self-transmission of the SXT/R391-like ICEs between P. vulgaris and Escherichia coli MG1655 strains.
Our results will facilitate further research on collaborative selection mechanism of environmental antibiotics and heavy
metals, and early warning in prevalence of novel MDR bacteria or “super bacteria”.

Key words: integrative and conjugative elements (ICEs), SXT/R391 family, multiple-drug resistance, heavy metal tolerance

中图分类号:

TS201.3

孙凤娇，贺羽，陈兰明*. 携带SXT/R391家族整合接合元件多重耐药菌株的高效筛选与分析[J]. 食品科学, doi: 10.7506/spkx1002-6630-201513016.

SUN Fengjiao, HE Yu, CHEN Lanming*. Effective Screening for Multiple-Drug Resistant Bacteria Harboring SXT/R391 Family of Integrative and Conjugative Elements[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201513016.

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携带SXT/R391家族整合接合元件多重耐药菌株的高效筛选与分析

Effective Screening for Multiple-Drug Resistant Bacteria Harboring SXT/R391 Family of Integrative and Conjugative Elements

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