复合PCR荧光检测方法检测转基因水稻品系

doi:10.7506/spkx1002-6630-201620032

食品科学 ›› 2016, Vol. 37 ›› Issue (20): 187-190.doi: 10.7506/spkx1002-6630-201620032

复合PCR荧光检测方法检测转基因水稻品系

张明哲，张晓峰，徐莉莉，陈笑梅，周圆，尹文秀，陈吴健，李孝军

1.浙江省检验检疫科学技术研究院，浙江杭州 310016；2.舟山出入境检验检疫局，浙江舟山 316000

收稿日期:2016-10-27 修回日期:2016-10-27 出版日期:2016-10-25 发布日期:2016-12-01
基金资助:
浙江省重大科技专项（2011C12023）；国家质检总局科技计划项目（2011IK249）；
浙江省公益技术项目（2011C22065；2014C32004） P

Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events

ZHANG Mingzhe, ZHANG Xiaofeng, XU Lili, CHEN Xiaomei, ZHOU Yuan, YIN Wenxiu, CHEN Wujian, LI Xiaojun

1. Zhejiang Academy of Science and Technology for Inspection and Quarantine, Hangzhou 310016, China;
2. Zhoushan Entry-Exit Inspection and Quarantine Bureau, Zhoushan 316000, China

Received:2016-10-27 Revised:2016-10-27 Online:2016-10-25 Published:2016-12-01

摘要/Abstract

摘要：

建立一种高通量的转基因复合聚合酶链式反应（polymerase chain reaction，PCR）荧光检测方法，该方法通过对转基因检测内源、外源及品系基因检测引物进行荧光标记，经复合PCR扩增和毛细管电泳荧光检测，实现特异性强、高通量检测。结果表明，复合PCR荧光检测方法检测4 种转基因水稻品系特异性好，并且灵敏度达到0.1%，可以在进出口检验检疫、食品安全监测等领域进行应用。

关键词: 复合聚合酶链式反应；毛细管电泳；转基因水稻；品系鉴定>

Abstract:

In this research, a high-throughput and specific method for the detection of genetically modified (GM) rice events
was developed using fluorescence multiplex PCR and capillary electrophoresis based on fluorescence-labeled primers for
endogenous, exogenous and event-specific genes. The fluorescence multiplex PCR method could specifically detect four
GM rice events with a sensitivity of 0.1%. This method has the potential to be applied in the inspection and quarantine of
import and export genetically modified commodities and in food safety detection.

Key words: multiplex PCR, capillary electrophoresis, genetic modified rice, identification of genetically modified events

中图分类号:

Q789h-

张明哲，张晓峰，徐莉莉，陈笑梅，周圆，尹文秀，陈吴健，李孝军. 复合PCR荧光检测方法检测转基因水稻品系[J]. 食品科学, 2016, 37(20): 187-190.

ZHANG Mingzhe, ZHANG Xiaofeng, XU Lili, CHEN Xiaomei, ZHOU Yuan, YIN Wenxiu, CHEN Wujian, LI Xiaojun. Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events[J]. FOOD SCIENCE, 2016, 37(20): 187-190.

复合PCR荧光检测方法检测转基因水稻品系

Establishment of a High-Throughput Fluorescence Multiplex PCR Method for Detection of Genetically Modified Rice Events

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价