食品科学 ›› 2020, Vol. 41 ›› Issue (6): 163-169.doi: 10.7506/spkx1002-6630-20190811-124

• 生物工程 • 上一篇    下一篇

瑞士乳杆菌MB2-1源胞外多糖对10 种益生菌生长特性的影响

黄蓉,张学亮,韩烁,周子文,莫乔雅,董明盛,芮昕,张秋勤,陈晓红,李伟   

  1. (南京农业大学食品科技学院,江苏 南京 210095)
  • 出版日期:2020-03-25 发布日期:2020-03-23
  • 基金资助:
    中央高校基本科研业务费专项(KYLH201702;KYYJ201807;KYYZ201904); 国家自然科学基金面上项目(31571818;31871771);NSFC-新疆联合基金项目(U1903108); 江苏省自然科学基金项目(BK20161448);江苏省“青蓝工程”项目; 南京农业大学SRT计划项目(国家级20181037051)

Effect of Exopolysaccharides of Lactobacillus helveticus MB2-1 on Growth Characteristics of Ten Probiotics

HUANG Rong, ZHANG Xueliang, HAN Shuo, ZHOU Ziwen, MO Qiaoya, DONG Mingsheng, RUI Xin, ZHANG Qiuqin, CHEN Xiaohong, LI Wei   

  1. (College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
  • Online:2020-03-25 Published:2020-03-23

摘要: 为研究瑞士乳杆菌(Lactobacillus helveticus)MB2-1源胞外多糖(exopolysaccharides,EPS)及其组分对益生菌生长特性的影响,更好阐明L. helveticus MB2-1源EPS的益生功能,测定10 种益生菌及2 株有害菌在以总EPS及其分离纯化后得到最多的组分EPS-2为唯一碳源的培养基中连续培养的光密度值(OD600 nm),并利用高效液相色谱法测定10 种益生菌在0~48 h中总EPS的剩余量及短链脂肪酸(short-chain fatty acids,SCFA)的生成量。结果表明,10 种益生菌在以源于L. helveticus MB2-1的总EPS和EPS-2纯组分为唯一碳源的培养基中,均可以利用总EPS和EPS-2以不同的速度生长繁殖,其中屎肠球菌(Enterococcus faecium)MN-1在第11.56小时最大比生长速率(μmax)达到0.225 h-1,而2 株有害菌的生长较在普通葡萄糖培养基中受到一定程度的抑制。在所有SCFA中,乳酸较其他SCFA生成量更大,其中乳酸生成量最多的是副干酪乳杆菌(L. paracasei),在第48小时浓度可达57.6 mmol/L,较发酵之初的26.1 mmol/L提高了121.7%。该研究表明L. helveticus MB2-1源EPS可作为10 种益生菌的良好碳源,也可以作为潜在的益生元,用于调节人体和动物的肠道健康。

关键词: 瑞士乳杆菌MB2-1, 胞外多糖, 生长曲线, 短链脂肪酸, 益生作用

Abstract: This study evaluated the effects of the exopolysaccharides (EPS) produced by Lactobacillus helveticus MB2-1 and its purified components on the growth characteristics of probiotics in order to gain further insights into the probiotic function of the EPS of L. helveticus MB2-1. The optical density (OD600 nm) values of ten probiotics and two pernicious strains continuously cultured in a medium with EPS or EPS-2, the most abundant component separated and purified from EPS, as the sole carbon source were determined. The residual amounts of total EPS and the amounts of short-chain fatty acids (SCFA) produced in 10 probiotics during 48 h of culture were measured by high performance liquid chromatography (HPLC). The results showed that the 10 probiotics could grow and reproduce at different rates in the medium with EPS or EPS-2 as the sole carbon source. Among them, Enterococcus faecium MN-1grown in EPS showed a maximum growth rate of 0.225 h-1 at 11.56 h. The growth of the two pernicious strains was inhibited to a certain extent compared with normal glucose medium. Lactic acid was produced in larger amounts than other SCFA. L. paracasei was the most efficient producer of lactic acid, and at 48 h of fermentation, the lactic acid concentration reached 57.6 mmol/L, which increased by 121.7% as compared with 26.1 mmol/L at the beginning of fermentation. This study demonstrated that the EPS derived from L. helveticus MB2-1 is a good carbon source for the ten probiotics and can be used as potential prebiotics to regulate intestinal health in humans and animals.

Key words: Lactobacillus helveticus MB2-1, exopolysaccharides, growth curve, short-chain fatty acids, probiotic function

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