食品科学 ›› 2021, Vol. 42 ›› Issue (10): 127-132.doi: 10.7506/spkx1002-6630-20200328-414

• 生物工程 • 上一篇    下一篇

鱼肠道中抗氧化活性乳酸菌的筛选及鉴定

丁丽丽,吕欣然,高永悦,崔晓玲,王小咪,白凤翎,仪淑敏,郭晓华   

  1. (1.渤海大学食品科学与工程学院,辽宁省食品安全重点实验室,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,国家鱼糜及鱼糜制品加工技术研发分中心,辽宁 锦州 121013;2.山东美佳集团有限公司,山东 日照 276815)
  • 出版日期:2021-05-25 发布日期:2021-06-02
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0901004);2019年“兴辽英才计划”青年拔尖人才项目(XLYC1907040)

Screening for and Identification of Lactic Acid Bacteria with Antioxidant Activity from the Intestinal Tract of Fish

DING Lili, LÜ Xinran, GAO Yongyue, CUI Xiaoling, WANG Xiaomi, BAI Fengling, YI Shumin, GUO Xiaohua   

  1. (1. Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, National R&D Branch Center of Surimi and Surimi Products Processing, College of Food Science & Engineering, Bohai University, Jinzhou 121013, China; 2. Shandong Meijia Group Co. Ltd., Rizhao 276815, China)
  • Online:2021-05-25 Published:2021-06-02

摘要: 从海水和淡水鱼肠道的乳酸菌中筛选具有抗氧化活性的乳酸菌菌株,旨在降低和预防水产品加工过程中氧化导致的质量问题。结果表明:从48 株乳酸菌中筛选出5 株具有较强抗氧化活性的乳酸菌,进一步复筛获得来源于草鱼肠道的菌株CY1-2的活性最强。菌株CY1-2对1,1-二苯基-2-三硝基苯肼自由基、羟自由基、超氧阴离子自由基清除率和抗脂质过氧化率分别为(67.29±0.42)%、(60.67±1.44)%、(29.87±1.14)%和(40.77±0.50)%,其无细胞提取物对羟自由基、超氧阴离子自由基清除率和抗脂质过氧化率分别为(64.27±1.26)%、(21.97±1.47)%和(51.03±0.40)%。此外,菌株CY1-2对革兰氏阳性菌地衣芽孢杆菌、金黄色葡萄球菌、蜡样芽孢杆菌和革兰氏阴性菌志贺氏菌、埃希氏大肠杆菌、铜绿假单胞菌及荧光假单胞菌均有抑菌活性。经生理生化和16S rDNA序列分析,菌株CY1-2被鉴定为植物乳杆菌(Lactobacillus plantarum)。植物乳杆菌CY1-2具有较强的抗氧化活性和广谱抑菌活性,对开发新型水产品天然微生物源性抗氧化剂具有一定的应用价值。

关键词: 鱼肠道;乳酸菌;抗氧化;抑菌;筛选与鉴定

Abstract: In this study, lactic acid bacteria (LAB) with antioxidant activity were isolated from the intestinal tract of seawater and freshwater fish for use in the prevention and attenuation of oxidative deterioration during the processing of aquatic products. The results were showed that five LAB strains with strong antioxidant activity were screened from the 48 LAB strains, among which strain CY1-2 from the intestinal tract of grass carps exhibited the strongest antioxidant activity. The antioxidant activity of the cells of this strain was determined to be (67.29 ± 0.42)%, (60.67 ± 1.44)% and (29.87 ± 1.14)% in the scavenging of 1,1-diphenyl-2-picryl-hydrazyl-hydrate (DPPH), hydroxyl and superoxide anion radicals and (40.77 ± 0.50)% in the inhibition of lipid peroxidation, respectively, and the percentages of hydroxyl and superoxide anion radical scavenging effect and anti-lipid peroxidation effect of the cell-free extract were (64.27 ± 1.26)%, (21.97 ± 1.47)%, and (51.03 ± 0.40)%, respectively. In addition, strain CY1-2 exhibited antibacterial activity against both gram-positive bacteria and gram-negative bacteria, including Bacillus licheniformis, Staphylococcus aureus, B. cereus, Shigella sp., Escherichia coli, Pseudomonas aeruginosa and Pseudomonas fluorescens. It was identified as Lactobacillus plantarum by physiological and biochemical characterization and 16S rDNA sequence analysis. Owing to its strong broad-spectrum antibacterial activity L. plantarum CY1-2 has the potential to be developed as a novel natural microorganism-derived antioxidant from aquatic products.

Key words: intestinal tract of fish; lactic acid bacteria; antioxidant; antimicrobial; screening and identification

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