基于非靶标代谢组学的沙棘油真实性鉴别技术

doi:10.7506/spkx1002-6630-20200327-393

食品科学 ›› 2021, Vol. 42 ›› Issue (18): 246-253.doi: 10.7506/spkx1002-6630-20200327-393

基于非靶标代谢组学的沙棘油真实性鉴别技术

徐冰冰，张九凯，赵贵明，韩建勋，胡静，邢冉冉，王玮，陈颖

（1.南京农业大学食品科技学院，江苏南京 210095；2.中国检验检疫科学研究院，北京 100176；3.石家庄工商职业学院，河北石家庄 050091）

发布日期:2021-09-29
基金资助:
“十三五”国家重点研发计划重点专项（2017YFF0211004；2018YFC1603504）

Authentication of Sea Buckthorn Oil Based on Non-targeted Metabolomics

XU Bingbing, ZHANG Jiukai, ZHAO Guiming, HAN Jianxun, HU Jing, XING Ranran, WANG Wei, CHEN Ying

(1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Chinese Academy of Inspection and Quarantine, Beijing 100176, China; 3. Shijiazhuang Vocational College of Industry and Commerce, Shijiazhuang 050091, China)

Published:2021-09-29

摘要/Abstract

摘要： 采用非靶标代谢组学的方法，基于超高效液相色谱-四极杆飞行时间质谱（ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry，UPLC-QTOF-MS）开展沙棘油真实性鉴别技术研究。化学计量学分析表明，通过正交偏最小二乘判别分析可以对沙棘油及其对照油（葵花籽油、菜籽油和大豆油）进行有效区分；根据离子丰度和质量数进一步筛选和鉴定，得到不同种类食用油的14 种特征标识化合物，包括有机酸、醇类等，其中沙棘油有6 种，葵花籽油有3 种，菜籽油有4 种，大豆油有1 种。结果表明，基于UPLC-QTOF-MS非靶标代谢组学的方法可以用于沙棘油及其对照油的有效鉴别。

关键词: 超高效液相色谱-四极杆飞行时间质谱；非靶标代谢组学；化学计量学；沙棘油；真实性鉴别

Abstract: In the present study, we examined the potential of non-targeted metabolomics based on ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) for the authentication of sea buckthorn oil. Chemometric analysis showed that sea buckthorn oil could be discriminated from other edible oils including sunflower seed oil, rapeseed oil and soybean oil by orthogonal partial least squares-discrimination analysis (OPLS-DA). Furthermore, 14 characteristic marker compounds in the oils were identified by their ion abundance and mass number, including organic acids and alcohols. Out of these compounds, six were found in sea buckthorn oil, three in sunflower seed oil, four in rapeseed oil and one in soybean oil. Our results suggest that non-targeted metabolomics based on UPLC-QTOF-MS has great potential for the rapid identification of adulterated sea buckthorn oil.

Key words: ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry; non-targeted metabolomics; chemometrics; sea buckthorn oil; authentication

中图分类号:

TS227

徐冰冰，张九凯，赵贵明，韩建勋，胡静，邢冉冉，王玮，陈颖. 基于非靶标代谢组学的沙棘油真实性鉴别技术[J]. 食品科学, 2021, 42(18): 246-253.

XU Bingbing, ZHANG Jiukai, ZHAO Guiming, HAN Jianxun, HU Jing, XING Ranran, WANG Wei, CHEN Ying. Authentication of Sea Buckthorn Oil Based on Non-targeted Metabolomics[J]. FOOD SCIENCE, 2021, 42(18): 246-253.

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基于非靶标代谢组学的沙棘油真实性鉴别技术

Authentication of Sea Buckthorn Oil Based on Non-targeted Metabolomics

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