蛹虫草转录组分析及类胡萝卜素生物合成相关基因的挖掘

doi:10.7506/spkx1002-6630-20200125-269

食品科学 ›› 2021, Vol. 42 ›› Issue (6): 150-156.doi: 10.7506/spkx1002-6630-20200125-269

蛹虫草转录组分析及类胡萝卜素生物合成相关基因的挖掘

娄海伟，赵玉，赵逸，林俊芳，赵仁勇，叶志伟，郭丽琼

（1.河南工业大学粮油食品学院，河南郑州 450001；2.华南农业大学食品学院，广东广州 510642；3.广东省微生态制剂工程技术研究中心，广东广州 510642）

出版日期:2021-03-25 发布日期:2021-03-29
基金资助:
广东省重点领域研发计划项目（2018B020205001；2018B020205003）；广东省现代农业食用菌产业技术体系项目（2019KJ103）；国家自然科学基金面上项目（31772373；31572178）；河南工业大学高层次人才科研基金项目（2020BS001）；国家自然科学基金-河南省联合基金重点项目（U1604234）

Transcriptomic Analysis of Cordyceps militaris and Mining of Genes Involved in Carotenoid Biosynthesis

LOU Haiwei, ZHAO Yu, ZHAO Yi, LIN Junfang, ZHAO Renyong, YE Zhiwei, GUO Liqiong

(1. College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China; 2. College of Food Science, South China Agricultural University, Guangzhou 510642, China; 3. Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province, Guangzhou 510642, China)

Online:2021-03-25 Published:2021-03-29

摘要/Abstract

摘要： 基于培养基显著影响蛹虫草类胡萝卜素的生成，采用高通量测序技术探究不同培养基培养的蛹虫草菌丝（CM10_RL和CM10_WL）的转录组差异，经生物信息学分析挖掘蛹虫草类胡萝卜素的生物合成相关基因。结果表明，样品CM10_WL的类胡萝卜素含量显著高于样品CM10_RL的类胡萝卜素含量；相对于样品CM10_RL，在样品CM10_WL中检测到318 个上调表达基因和618 个下调表达基因。基因本体论功能富集结果表明差异表达基因（differentially expressed gene，DEG）主要富集在代谢过程、细胞膜、催化活性条目中。京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集结果表明DEG主要富集在代谢途径。蛹虫草转录本与KEGG数据库中的类胡萝卜素生物合成途径比对结果表明基因CCM_06728和CCM_09155参与类胡萝卜素的生物合成。本研究为进一步阐明蛹虫草类胡萝卜素的生物合成途径奠定基础。

关键词: 蛹虫草；高通量测序；转录组；色素；类胡萝卜素；差异表达基因

Abstract: Culture medium composition significantly affects carotenoid biosynthesis in Cordyceps militaris. In this study, high-throughput sequencing technology was used to investigate the transcriptomic differences of C. militaris mycelia (CM10_RL and CM10_WL) cultured with different media. The genes related to the biosynthesis of carotenoids in C. militaris were identified by bioinformatics analysis. The results showed that the carotenoid content of CM10_WL was significantly higher than that of CM10_RL. A total of 318 up-regulated genes and 618 down-regulated genes were found in CM10_WL compared with CM10_RL. Gene Ontology (GO) analysis indicated that the differentially expressed genes (DEGs) were mainly classified into the “metabolic process”, “membrane” or “catalytic activity” terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis suggested that these DEGs were mainly enriched in “metabolic pathways”. Therefore, all the transcripts derived from RNA-seq data were searched against the carotenoid biosynthetic pathway in the KEGG database, and the results showed that the CCM_06728 and CCM_09155 genes were involved in the biosynthesis of carotenoids. This study lay a foundation for further elucidating the biosynthetic pathway of carotenoids in C. militaris.

Key words: Cordyceps militaris; high-throughput sequencing; transcriptome; pigment; carotenoid; differentially expressed gene

中图分类号:

TS201.3

娄海伟，赵玉，赵逸，林俊芳，赵仁勇，叶志伟，郭丽琼. 蛹虫草转录组分析及类胡萝卜素生物合成相关基因的挖掘[J]. 食品科学, 2021, 42(6): 150-156.

LOU Haiwei, ZHAO Yu, ZHAO Yi, LIN Junfang, ZHAO Renyong, YE Zhiwei, GUO Liqiong. Transcriptomic Analysis of Cordyceps militaris and Mining of Genes Involved in Carotenoid Biosynthesis[J]. FOOD SCIENCE, 2021, 42(6): 150-156.

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蛹虫草转录组分析及类胡萝卜素生物合成相关基因的挖掘

Transcriptomic Analysis of Cordyceps militaris and Mining of Genes Involved in Carotenoid Biosynthesis

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