基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性

doi:10.7506/spkx1002-6630-20200302-024

食品科学 ›› 2021, Vol. 42 ›› Issue (7): 142-148.doi: 10.7506/spkx1002-6630-20200302-024

基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性

詹慧，宋田源，余刚，姜泽东，杜希萍，朱艳冰，倪辉，李清彪

（1.集美大学食品与生物工程学院，福建厦门 361021；2.福建省食品微生物与酶工程重点实验室，福建厦门 361021；3.厦门南方海洋研究中心经济海藻资源化利用与深加工重点实验室，福建厦门 361021；4.厦门市食品生物工程技术研究中心，福建厦门 361021）

出版日期:2021-04-15 发布日期:2021-05-17
基金资助:
国家自然科学基金面上项目（31972081）；福建省自然科学基金重点项目（2018N0019）；福建省自然科学基金面上项目（2019J01697）

Hypolipidemic Activity of Polysaccharides Purified from Bangia fusco-purpurea

ZHAN Hui, SONG Tianyuan, YU Gang, JIANG Zedong, DU Xiping, ZHU Yanbing, NI Hui, LI Qingbiao

(1. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; 2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; 3. Key Laboratory of Systemic Utilization and In-depth Processing of Economic Seaweed, Xiamen Southern Oceangraphic Center, Xiamen 361021, China; 4. Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China)

Online:2021-04-15 Published:2021-05-17

摘要/Abstract

摘要： 目的：对红毛藻（Bangia fusco-purpurea）中多糖组分进行分离纯化，分析多糖组分的体外降血脂活性，阐明红毛藻的降血脂活性功效机理，为红毛藻的精深加工和高值化应用提供科学依据。方法：采用热水提取-乙醇沉淀法从红毛藻中提取粗多糖；除去蛋白质后，通过Sephadex G75葡聚糖凝胶柱层析纯化后得到红毛藻多糖（B. fusco-purpurea polysaccharide，BFP）；采用DEAE-cellulose 52柱层析对BFP进一步分级纯化得到3 个多糖组分F1、F2和F3；通过酶动力学分析各组分对胰脂肪酶活性的影响；采用噻唑蓝法测定BFP组分F1、F2和F3对HepG2细胞和Caco-2细胞活力的影响；通过高血脂/高胆固醇HepG2细胞模型、油酸诱导Caco-2细胞模型分析BFP组分F1、F2和F3的体外降血脂活性。结果：酶动力学分析结果表明，BFP组分F3显著抑制了胰脂肪酶活性，且为可逆竞争性抑制；细胞模型结果表明，F1、F2和F3在质量浓度0～500 mg/mL范围内对所试细胞均无显著毒性作用；F1显著抑制Caco-2细胞对游离脂肪酸的吸收；F3对高血脂HepG2细胞模型中甘油三酯的合成和高胆固醇HepG2细胞模型中脂类的合成均有显著抑制作用。结论：BFP组分可有效抑制胰脂肪酶活性和细胞对游离脂肪酸的吸收、降低胆固醇和甘油三酯的合成，具有潜在的降血脂活性。

关键词: 红毛藻多糖；胰脂肪酶；抑制作用；降血脂活性

Abstract: Objective: To evaluate the in vitro hypolipidemic activity of three sulfated polysaccharide fractions purified from the edible red alga Bangia fusco-purpurea for the purpose of providing a scientific basis for deep processing and high-valued utilization of Bangia fusco-purpurea. Methods: The crude polysaccharide was prepared by hot water extraction followed by 75% ethanol precipitation, deproteinized with the Savage method, purified by Sephadex G75 column chromatography, and fractionated by DEAE-cellulose 52 column chromatography into three fractions (F1, F2 and F3). The inhibitory effects of the polysaccharides on pancreatic lipase were analyzed, and the effect on the viability of Caco-2 and HepG2 cells was measured by the MTT assay. The in vitro hypolipidemic potentials were evaluated using HepG2 cell model and Caco-2 cell model. Results: F3 significantly inhibited pancreatic lipase activity in a competitive and reversible manner. All three fractions showed no significant cytotoxicity at tested concentrations (0–500 mg/mL). Interestingly, F1 significantly inhibited the absorption of free fatty acids by Caco-2 cells, whereas F3 exhibited potent inhibitory effects on triglyceride synthesis in the hyperlipidemic HepG2 cell model and lipid synthesis in the high cholesterol HepG2 cell model. Conclusion: The polysaccharides from B. fusco-purpurea can effectively inhibit pancreatic lipase activity, and obviously suppress the absorption of free fatty acids by cells and the synthesis of cholesterol and triglycerides in cells, thereby exhibiting hypolipidemic potentials.

Key words: Bangia fusco-purpurea polysaccharide; pancreatic lipase; inhibitory effect; hypolipidemic activity

中图分类号:

TS201.2

詹慧，宋田源，余刚，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性[J]. 食品科学, 2021, 42(7): 142-148.

ZHAN Hui, SONG Tianyuan, YU Gang, JIANG Zedong, DU Xiping, ZHU Yanbing, NI Hui, LI Qingbiao. Hypolipidemic Activity of Polysaccharides Purified from Bangia fusco-purpurea[J]. FOOD SCIENCE, 2021, 42(7): 142-148.

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基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性

Hypolipidemic Activity of Polysaccharides Purified from Bangia fusco-purpurea

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