植物乳杆菌LP21绿色合成纳米硒及对溶藻弧菌的抑菌活性

doi:10.7506/spkx1002-6630-20210104-026

食品科学 ›› 2022, Vol. 43 ›› Issue (2): 217-223.doi: 10.7506/spkx1002-6630-20210104-026

植物乳杆菌LP21绿色合成纳米硒及对溶藻弧菌的抑菌活性

王丽红，杨辉，苏文，孔阳

（陕西科技大学食品与生物工程学院，陕西西安 710021）

出版日期:2022-01-25 发布日期:2022-01-29
基金资助:
陕西省科技厅技术创新引导专项（2017CG-003）；陕西省重点研发计划项目（2018NY-043）

Biosynthesis of Nano-Selenium by Lactobacillus plantarum LP21 and Its Antibacterial Activity against Vibrio alginolyticus

WANG Lihong, YANG Hui, SU Wen, KONG Yang

(School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi’an 710021, China)

Online:2022-01-25 Published:2022-01-29

摘要/Abstract

摘要： 采用益生菌植物乳杆菌LP21合成纳米硒，为抑制水产养殖中溶藻弧菌感染提供一种新的方法。通过植物乳杆菌LP21绿色合成纳米硒，对所得纳米硒进行透射电镜形貌及能谱分析，粒径仪测定粒径，红外光谱分析纳米硒表面生物分子的分布，并研究纳米硒对溶藻弧菌的抑菌活性。结果表明，植物乳杆菌LP21还原的纳米硒呈球形，分散性良好；纯化的纳米硒平均尺寸为184.6 nm，其表面包裹蛋白、多糖等生物分子，呈无定形态；纳米硒对溶藻弧菌、大肠杆菌的抑菌活性较强，对溶藻弧菌的最小抑菌浓度为286 μg/mL，在亚抑制剂量下对溶藻弧菌的生长、运动性、生物膜有显著抑制，并引起菌体细胞形态发生变化，此抑菌研究为纳米硒在水产养殖业感染菌防治中的应用提供依据。

关键词: 植物乳杆菌；纳米硒；溶藻弧菌；结构表征；抑菌活性

Abstract: Nano-selenium synthesized by the probiotic bacterium Lactobacillus plantarum LP21 was analyzed by transmission electron microscopy (TEM) and energy spectroscopy, and its particle size was measured by a particle size analyzer. Additionally, biomolecule distribution on the surface of nano-selenium were analyzed by Fourier transform infrared (FTIR) spectroscopy, and the antibacterial activity of nano-selenium against Vibrio alginolyticus was tested. The results showed that the nano-selenium was spherical in shape and well dispersed; the average particle size of the purified nano-selenium was 184.6 nm, and its surface was covered with proteins, polysaccharides and other biological molecules, showing an amorphous form. The antibacterial activity of nano-selenium against V. alginolyticus and Escherichia coli was strong, and the minimum inhibitory concentration (MIC) against V. alginolyticus was 286 μg/mL. The growth, motility and biofilm of V. alginolyticus were significantly inhibited by the nano-selenium at sub-inhibitory doses, and the cell morphology was changed as well. The results of this study provide a basis for its application to control pathogenic infection in aquaculture.

Key words: Lactobacillus plantarum; nano-selenium; Vibrio alginolyticus; structural characterization; antibacterial activity

中图分类号:

TS201.3

王丽红，杨辉，苏文，孔阳. 植物乳杆菌LP21绿色合成纳米硒及对溶藻弧菌的抑菌活性[J]. 食品科学, 2022, 43(2): 217-223.

WANG Lihong, YANG Hui, SU Wen, KONG Yang. Biosynthesis of Nano-Selenium by Lactobacillus plantarum LP21 and Its Antibacterial Activity against Vibrio alginolyticus[J]. FOOD SCIENCE, 2022, 43(2): 217-223.

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植物乳杆菌LP21绿色合成纳米硒及对溶藻弧菌的抑菌活性

Biosynthesis of Nano-Selenium by Lactobacillus plantarum LP21 and Its Antibacterial Activity against Vibrio alginolyticus

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