木犀草苷对3T3-L1前脂肪细胞分化和脂代谢的影响

doi:10.7506/spkx1002-6630-20240117-155

食品科学 ›› 2024, Vol. 45 ›› Issue (18): 116-123.doi: 10.7506/spkx1002-6630-20240117-155

木犀草苷对3T3-L1前脂肪细胞分化和脂代谢的影响

杨梦, 任子怡, 米思, 冯丹琦, 程鑫颖, 冯雪, 刘卫华, 王向红

（1.河北农业大学食品科技学院，河北保定 071000；2.河北晨光检测技术服务有限公司，河北邯郸 056107）

出版日期:2024-09-25 发布日期:2024-09-09
基金资助:
河北省自然科学基金项目（C2022204224）

Effect of Luteoloside on 3T3-L1 Preadipocyte Differentiation and Lipid Metabolism

YANG Meng, REN Ziyi, MI Si, FENG Danqi, CHENG Xinying, FENG Xue, LIU Weihua, WANG Xianghong

(1. College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China;2. Hebei Chenguang Detection Technology Service Co. Ltd., Handan 056107, China)

Online:2024-09-25 Published:2024-09-09

摘要/Abstract

摘要： 目的：研究木犀草苷对3T3-L1前脂肪细胞分化和脂代谢的影响及其作用机制。方法：采用3T3-L1前脂肪细胞，利用四甲基偶氮唑蓝法检测不同质量浓度木犀草苷对3T3-L1前脂肪细胞毒性的影响；使用鸡尾酒诱导法诱导细胞分化，用油红O染色观察脂滴形成情况；测定分化后细胞中甘油三酯、总胆固醇的含量以及相关炎症因子如肿瘤坏死因子（tumor necrosis factor，TNF）-α、白细胞介素（interleukin，IL）-6、IL-10、瘦素（leptin，LEP）和脂联素（adiponectin，ADPN）的分泌量；利用Western Blot法检测细胞过氧化物酶体增殖物激活受体（peroxisome proliferators activated receptor，PPAR）γ、CCAAT增强子结合蛋白（CCAAT-enhancer-binding proteins，C/EBP）-α、固醇调节元件结合蛋白1（sterol regulatory element-binding protein 1，SREBP1）、PPARα、解偶联蛋白1（uncoupling protein 1，UCP-1）、肉碱棕榈酰转移酶1（carnitine palmitoyltransferase 1，CPT-1）蛋白的相对表达水平。结果：3T3-L1前脂肪细胞存活率随木犀草苷质量浓度的升高整体呈降低趋势，木犀草苷质量浓度在5～60 μg/mL时细胞存活率均在80%以上。木犀草苷处理组可显著抑制3T3-L1前脂肪细胞的分化，减少脂质积累；木犀草苷可下调成脂分化后细胞中总胆固醇、甘油三酯的含量以及炎症因子TNF-α、IL-6和LEP的分泌量，上调IL-10和ADPN的分泌量；木犀草苷能下调PPARγ、C/EBP-α和SREBP1蛋白的相对表达水平，上调PPARα、UCP-1和CPT-1蛋白的相对表达水平。结论：木犀草苷可以抑制3T3-L1前脂肪细胞分化和影响脂代谢，其机制一方面是下调PPARγ、C/EBP-α和SREBP1蛋白表达，抑制脂肪合成；另一方面是激活PPARα上调下游蛋白，促进脂肪消耗；木犀草苷还可能通过调节细胞因子的分泌量，促进细胞脂解，从而改善脂代谢失衡。

关键词: 木犀草苷；3T3-L1前脂肪细胞；分化；脂代谢；肥胖

Abstract: Objective: To study the effect and mechanism of luteoloside on the differentiation and lipid metabolism of 3T3-L1 preadipocytes. Methods: The cytotoxicity of different concentrations of luteoloside on 3T3-L1 preadipocyte was detected using methyl thiazolyl tetrazolium (MTT) assay. Cell differentiation was induced by the cocktail method, and lipid droplets were observed by oil red O staining. The contents of triglyceride and total cholesterol in differentiated cells, as well as the secretion of related inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-10, leptin (LEP) and Adiponectin (ADPN) were measured. The relative expression levels of peroxisome proliferators activated receptor (PPAR) γ, CCAAT-enhancer-binding proteins (C/EBP)-α, sterol regulatory element-binding protein 1 (SREBP1), PPARα, uncoupling protein 1 (UCP-1), and carnitine palmitoyltransferase 1 (CPT-1) were analyzed by Western blotting. Results: The survival rate of 3T3-L1 preadipocytes decreased with the increase in luteoloside concentration, being above 80% at luteoloside concentrations of 5–60 μg/mL. Treatment with luteoloside significantly inhibited the differentiation of 3T3-L1 preadipocytes and reduced lipid accumulation. Luteoloside down-regulated the contents of TC and TG, the secretion of TNF-α, IL-6 and LEP, but up-regulated the secretion of IL-10 and ADPN. Luteoloside down-regulated the protein expression levels of PPARγ, C/EBP-α and SREBP1, but up-regulated those of PPARα, UCP-1 and CPT-1. Conclusion: Luteoloside can suppression the differentiation and lipid metabolism of 3T3-L1 preadipocytes. The mechanism may be that luteoloside down-regulates the protein expression of PPARγ, C/EBP-α, and SREBP1 to inhibit fat synthesis. Furthermore, it activates PPARα to upregulate downstream proteins and promote fat consumption. Luteoloside may also regulate the secretion of cytokines and promote cellular lipolysis, thus improving lipid metabolism imbalance.

Key words: luteoloside; 3T3-L1 preadipocytes; differentiation; lipid metabolism; obesity

中图分类号:

TS201.4

杨梦, 任子怡, 米思, 冯丹琦, 程鑫颖, 冯雪, 刘卫华, 王向红. 木犀草苷对3T3-L1前脂肪细胞分化和脂代谢的影响[J]. 食品科学, 2024, 45(18): 116-123.

YANG Meng, REN Ziyi, MI Si, FENG Danqi, CHENG Xinying, FENG Xue, LIU Weihua, WANG Xianghong. Effect of Luteoloside on 3T3-L1 Preadipocyte Differentiation and Lipid Metabolism[J]. FOOD SCIENCE, 2024, 45(18): 116-123.

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木犀草苷对3T3-L1前脂肪细胞分化和脂代谢的影响

Effect of Luteoloside on 3T3-L1 Preadipocyte Differentiation and Lipid Metabolism

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