食品科学 ›› 2009, Vol. 30 ›› Issue (22): 162-164.doi: 10.7506/spkx1002-6300-200922035

• 工艺技术 • 上一篇    下一篇

猪胰脏中胰酶的提取工艺优化研究

郭兆斌1,韩 玲1,*,刘亮亮1,李君兰1,2   

  1. 1.甘肃农业大学食品科学与工程学院 2.河西学院农学系
  • 收稿日期:2008-12-19 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 韩 玲1,*, E-mail:hanl@gsau.edu.cn
  • 基金资助:

    甘肃省农业生物技术研究开发项目(GNSW-2007-06)

Extraction of Pancreatin from Pig Pancreas

GUO Zhao-bin1,HAN Ling1,*,LIU Liang-liang1,LI Jun-lan1,2   

  1. (1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
    2. Department of Agronomy, Hexi University, Zhangye 734000, China)
  • Received:2008-12-19 Online:2009-11-15 Published:2010-12-29
  • Contact: HAN Ling1,*, E-mail:hanl@gsau.edu.cn

摘要:

目的:采用异丙醇为提取剂,一种新型沉淀剂壳聚糖对猪胰脏中胰酶的制备工艺进行研究。方法:通过单因素试验和正交试验,对影响胰酶收率和活力的因素(异丙醇浓度、提取时间、pH 值、壳聚糖用量)进行研究,确定最佳工艺条件。结果:所得的最佳工艺条件为异丙醇浓度7.5%、提取时间6h、pH4、壳聚糖用量0.1%。结论:所制得的胰酶收率为12.19%,胰蛋白酶、胰淀粉酶和胰脂肪酶的酶活分别为4836、14320、20580U/g,说明壳聚糖有很好的沉淀絮凝效果。

关键词: 猪胰脏, 壳聚糖, 胰酶, 提取

Abstract:

Pancreatin was extracted from pig pancreas using isopropyl alcohol as solvent and chitosan as precipitant. The effect of isopropyl alcohol concentration, pH, extraction time and amount of chitosan on the yield and activities of pancreatin was stuided by conducting single factor and the following orthogonal test. The optimum extraction conditions were that pig pancreas was extracted with 7.5% isopropyl at pH 4 for 6 h, and pancreatin was precipitated with 0.1% chitosan. Under such conditions, the yield of pancreatin was 12.19%, and the activities of trypsin, amylopsin and lipase in it was 4836, 14320, 20580 U/g respectively. Chitosan, as a new precipitan, was effective in the extraction of pancreatin.

Key words: pig pancreas, chitosan, pancreatin, extraction

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