食品科学 ›› 2009, Vol. 30 ›› Issue (22): 140-145.doi: 10.7506/spkx1002-6300-200922030

• 工艺技术 • 上一篇    下一篇

挤压膨化预处理水酶法提取大豆蛋白的工艺研究

李 杨,江连洲* ,许 晶,胡少新,杨 柳   

  1. 东北农业大学食品学院
  • 收稿日期:2008-12-23 出版日期:2009-11-15 发布日期:2010-12-29
  • 通讯作者: 江连洲* E-mail:jlzname@163.com
  • 基金资助:

    农业部现代大豆产业技术体系建设项目(nycytx-004)

Extraction of Soybean Protein through Enzymatic Hydrolysis Combined with Pretreatment of Extrusion

LI Yang,JIANG Lian-zhou*,XU Jing,HU Shao-xin,YANG Liu   

  1. (College of Food Science, Northeast Agricultural University, Harbin 150030, China)
  • Received:2008-12-23 Online:2009-11-15 Published:2010-12-29
  • Contact: JIANG Lian-zhou*, E-mail:jlzname@163.com

摘要:

采用水酶法结合挤压膨化预处理提取大豆蛋白,筛选5 种蛋白酶,确定选用碱性蛋白酶作为水解酶;得出碱性蛋白酶提取大豆蛋白的最佳条件:加酶量1.9%、酶解温度50℃、酶解时间200min、料水比1:4.6、酶解pH8.5,经过验证与对比实验可知在最优酶解工艺条件下总蛋白提取率可达到93.76%左右,比传统的湿热预处理后酶解的总蛋白提取率78.83% 提高了近15 个百分点。

关键词: 水酶法, 挤压膨化, 大豆蛋白

Abstract:

Enzymatic hydrolysis combined with pretreatment of extrusion was used to extract soybean protein. An alkaline proteinase has been screened from 5 proteinases. The optimal condition for soybean protein extraction by alkaline proteinase was 1.9% proteinase, reaction at 50 ℃, 3.3 h of hydrolysis, 1:4.6 of material and water ratio, and pH 8.5. The total protein extraction rate reached to 93.76% under this optimal condition, which was nearly 15% higher than total protein extraction using enzymatic hydrolysis combined with traditional treatment of heating.

Key words: enzymatic hydrolysis method, extrusion, soybean protein

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