食品科学 ›› 2009, Vol. 30 ›› Issue (12): 69-72.doi: 10.7506/spkx1002-6630-200912010

• 工艺技术 • 上一篇    下一篇

超声辅助溶剂法提取白簕叶总皂苷的工艺研究

高 侠1,黎云祥1,*,蔡凌云1,2,王 荣1, 权秋梅1,陈 蕉1   

  1. 1.西华师范大学生命科学学院,四川省高校环境科学与生物多样性保护重点实验室 2.凯里学院化学系
  • 收稿日期:2008-09-23 修回日期:2009-02-04 出版日期:2009-06-15 发布日期:2010-12-29
  • 通讯作者: 黎云祥1,*, E-mail:yxli@163.com
  • 基金资助:

    四川省教育厅重点项目(2006A078);四川省重点学科项目(SZD0420)

Ultrasonic-assisted Solvent Extraction of Total Saponins from A. trifoliatus Leaves

GAO Xia1,LI Yun-xiang1,*,CAI Ling-yun1,2,WANG Rong1,QUAN Qiu-mei1,CHEN Jiao1   

  1. (1. Key Laboratory of Environmental Science and Biodiversity Conservation of Sichuan Higher Education, College of Life Science, China
    West Normal University, Nanchong 637002, China;2. Department of Chemistry, Kaili University, Kaili 556000, China)
  • Received:2008-09-23 Revised:2009-02-04 Online:2009-06-15 Published:2010-12-29
  • Contact: LI Yun-xiang1,*, E-mail:yxli@163.com

摘要:

以白簕叶总皂苷提取率为评价指标,对超声波法提取分离白簕叶总皂苷进行了实验研究。考察了超声时间、超声功率、超声温度这三因素对总皂苷提取率的影响,并在单因素考察基础上,选用L9(34)正交表进行正交试验。结果表明 :白簕叶总皂苷超声提取的最佳工艺条件为:超声时间35min,超声温度为60℃,超声功率80%,料液比为1:10,该条件下白簕叶总皂苷提取率为16.32%,高于索氏提取总皂苷提取率。

关键词: 超声, 总皂苷, 白簕, 提取

Abstract:

In the present study, ultrasonic treatment was applied to assist the extraction of total saponins from defatted and decolorized A. trifoliatus leaves with 70% ethanol. The ultrasonic-assisted extraction technology was optimized by orthogonal test design based on single factor test. Results showed that the optimal ultrasonic power, ultrasonic treatment duration, extraction temperature and ratio of solid to liquid for the extraction of total saponins were 80% (relative value to the maximum power), 35 min, 60 ℃ and 1:10; under this condition, the extraction yield of total saponins was 16.32%, which was higher than that obtained by Soxhlet extraction.

Key words: ultrasonic, total saponins, A. trifoliatus, extraction

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