食品科学 ›› 2011, Vol. 32 ›› Issue (5 ): 172-175.doi: 10.7506/spkx1002-6630-201105037

• 生物工程 • 上一篇    下一篇

中国金顶侧耳菌株遗传多态性的ISSR分析

张秋胜1,徐丙莲2,刘林德1,程显好1,高兴喜1,史亚丽3   

  1. 1. 鲁东大学生命科学学院
    2. 鲁东大学数学与信息学院
    3.鲁东大学体育学院
  • 收稿日期:2010-05-18 修回日期:2011-01-21 出版日期:2011-03-15 发布日期:2011-03-03
  • 通讯作者: 张秋胜 E-mail:qiushengzhang@126.com
  • 基金资助:
    “十一五”国家科技支撑计划项目(2008BADA1B04);鲁东大学人才基金项目(LY20063303);
    鲁东大学创新团队建设项目;鲁东大学学科建设经费项目

Genetic Polymorphism Analysis of Pleurotus cituinopileatus by ISSR

ZHANG Qiu-sheng1,XU Bing-lian2,LIU Lin-de1,CHENG Xian-hao1,GAO Xing-xi1,SHI Ya-li   

  1. 1. School of Life Science, Ludong University, Yantai 264025, China;2. School of Mathmatics and Information, Ludong
    University, Yantai 264025, China;3. Sports Institute, Ludong University, Yantai 264025, China
  • Received:2010-05-18 Revised:2011-01-21 Online:2011-03-15 Published:2011-03-03
  • Contact: ZHANG Qiu-sheng E-mail:qiushengzhang@126.com

摘要: 金顶侧耳是重要的食药用真菌。本实验收集全国各地18种金顶侧耳菌株,通过ISSR分子标记技术分析金顶侧耳菌株种内遗传多态性,11种引物扩增出102个条带,其中78个条带具有多态性,多态率为76.4%。结果表明,金顶侧耳菌株内具有丰富的遗传多样性,遗传相似系数在0.50~0.96。采用平均分类法UPGMA分析表明,在遗传相似系数为0.62时,18种菌株分为两大类。当遗传相似系数增大到0.75时,它们分为7类。16号菌株具有独立的遗传体系。

关键词: 金顶侧耳, 简短重复序列, 多态性, 亲缘关系

Abstract: Pleurotus cituinopileatus is an important kind of edible medicinal fungi. Totally 18 kinds of Pleurotus cituinopileatus strains were collected from some markets in China. Inter-simple sequence repeat (ISSR) method was used to analyze genetic polymorphism of Pleurotus cituinopileatus strains. A total of 102 DNA fragments were amplified by using eleven ISSR primers, and 78 of these fragments were polymorphic with polymorphism rate of 76.4%. Meanwhile, high-level genetic polymorphism was observed in Pleurotus cituinopileatus and the genetic similarity coefficients (GSC) were between  0.50 and 0.96. These 18 strains were divided into 2 groups at GSC of 0.62 and 7 groups at GSC of 0.75 according to the cluster analysis of UPGMA method. Strain 16 had an independent genetic system. These results will provide a scientific fundament to accelerate genetic breeding and identification of cultivated strains of Pleurotus cituinopileatus.

Key words: Pleurotus cituinopileatus, inter-simple sequence repeat (ISSR), polymorphism, intra-specific relationship

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