响应面法优化桑黄液体发酵培养基

doi:10.7506/spkx1002-6630-201107048

食品科学 ›› 2011, Vol. 32 ›› Issue (7 ): 224-228.doi: 10.7506/spkx1002-6630-201107048

响应面法优化桑黄液体发酵培养基

谢丽源，谭伟，郭勇，贾定洪，彭卫红，甘炳成*

四川省农业科学院土壤肥料研究所

收稿日期:2010-06-09 修回日期:2011-02-23 出版日期:2011-04-15 发布日期:2011-03-30
通讯作者: 谢丽源 E-mail:xieliyuan77@163.com
基金资助:
四川省青年基金项目(2008ZQ026-072);四川省科技支撑计划项目(2008FZ0157)

Application of Response Surface Methodology for Optimization of Liquid Fermentation Medium for Intracellular Polysaccharide Production by Phellinus baumii

XIE Li-yuan,TAN Wei,GUO Yong,JIA Ding-hong,PENG Wei-hong,GAN Bing-cheng

Institute of Soil and Fertilizer,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China

Received:2010-06-09 Revised:2011-02-23 Online:2011-04-15 Published:2011-03-30

摘要/Abstract

摘要： 利用响应面分析法对桑黄菌丝体生物量及产胞内多糖的液体发酵培养基进行优化，研究碳源、氮源、无机盐对桑黄菌丝生物量、胞内多糖含量及产量的影响。在单因素筛选试验的基础上，利用Box-Benhnken设计和响应面分析法对碳源、氮源和无机盐水平进行分析。结果表明，桑黄产胞内多糖的液体发酵培养基最佳组合为：玉米粉3.9%、麸皮2.2%、KH2PO4 0.20%、MgSO4 0.10%，在此条件下的验证实验表明，胞内多糖产量可达233.107mg/L。

关键词: 桑黄, 液体发酵, 培养基, 胞内多糖, 响应面分析法

Abstract: The objective of the present study was the optimization of liquid fermentation medium for producing intracellular polysaccharide by Phellinus baumii by response surface methodology. The effects of carbon source, nitrogen source, and metal ions on mycelium biomass and intracellular polysaccharide production were explored in single factor experiments, and corn flour and wheat bran were found to be the best carbon source and nitrogen source, respectively. Based on this, the optimum levels of corn flour, wheat bran, KH2PO4 and MgSO4 were determined by Box-Benhnken design combined with response surface analysis to be 3.9%, 2.2%, 0.20% and 0.10%, respectively. The intracellular polysaccharide production in optimized medium reached 233.107 mg/L.

Key words: Phellinus baumii, liquid-state fermentation, culture medium, intracellular polysaccharide, response surface methodology

中图分类号:

TQ920.1

谢丽源，谭伟，郭勇，贾定洪，彭卫红，甘炳成. 响应面法优化桑黄液体发酵培养基[J]. 食品科学, 2011, 32(7 ): 224-228.

XIE Li-yuan,TAN Wei,GUO Yong,JIA Ding-hong,PENG Wei-hong,GAN Bing-cheng. Application of Response Surface Methodology for Optimization of Liquid Fermentation Medium for Intracellular Polysaccharide Production by Phellinus baumii[J]. FOOD SCIENCE, 2011, 32(7 ): 224-228.

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响应面法优化桑黄液体发酵培养基

Application of Response Surface Methodology for Optimization of Liquid Fermentation Medium for Intracellular Polysaccharide Production by Phellinus baumii

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