食品科学 ›› 2011, Vol. 32 ›› Issue (2 ): 127-131.doi: 10.7506/spkx1002-6630-201102030

• 工艺技术 • 上一篇    下一篇

响应面法优化火麻仁黄酮提取工艺

赵翾1,李红良2,张惠妹1   

  1. 1.仲恺农业工程学院轻工食品学院 2.东莞市广益食品添加剂实业有限公司
  • 收稿日期:2010-11-05 出版日期:2011-01-25 发布日期:2011-01-10
  • 通讯作者: 赵翾 E-mail:zxzhaoxuan@163.com

Optimization of Flavonoids Extraction from Fructus Cannabis by Response Surface Methodology

ZHAO Xuan1,LI Hong-liang2,ZHANG Hui-mei1   

  1. 1. College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;
    2. Dongguan Guangyi Food Additive Industry Co. Ltd., Dongguan 523000, China
  • Received:2010-11-05 Online:2011-01-25 Published:2011-01-10
  • Contact: ZHAO Xuan1 E-mail:zxzhaoxuan@163.com

摘要:

对火麻仁黄酮的提取工艺进行研究。通过单因素试验分别考察提取溶剂、乙醇体积分数、浸提时间、浸提温度、液料比和粉碎度对火麻仁黄酮提取量的影响,确定各因素的适宜水平。在此基础上,利用Design-Expert软件中心组合设计法设计响应面试验,并通过方差分析回归建立数学模型,得到火麻仁黄酮的最佳提取工艺条件为乙醇体积分数70%、提取温度75℃、提取时间2.5h、液料比35:1(mL/g),在此条件下,黄酮提取量为17.80mg/g。

关键词: 火麻仁, 黄酮, 响应面分析, 工艺优化

Abstract:

To optimize the process for flavnonoids extraction from Fructus Cannabis, a series of single-factor experiments were made to investigate the effects of extraction solvent type, ethanol concentration, extraction duration and temperature, liquid-tosolid ratio ratio and the degree of pulverization of the raw material on flavnonoids yield, and based on this, a 19-run response surface design involving 5 of the above 6 factors at 3 levels was generated by the Design-Expert software according to the central composite design principle and experimental data obtained were subjected to quadratic regression analysis to create a mathematical model describing flavnonoids extraction. The optimal extraction conditions were found to be: ethanol concentration of 70% and liquid-to-solid ratio of 35:1 for an extraction duration of 2.5 h at 75 ℃. Under these conditions, the yield of flavonoids was 17.80 mg/g.

Key words: Fructus Cannabis, flavonoid, response surface methodology, technical optimization

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