食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 188-192.doi: 10.7506/spkx1002-6630-200924040

• 工艺技术 • 上一篇    下一篇

响应曲面法优化动物蛋白酶水解脱脂蚕蛹蛋白的工艺

张海祥1,魏兆军1 ,* ,周乐春1,廖爱美1 , 2   

  1. 1.合肥工业大学生物与食品工程学院 2.合肥师范学院生命科学系
  • 收稿日期:2009-08-12 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 魏兆军1 ,* E-mail:zjwei@hfut.edu.cn
  • 基金资助:

    安徽省“十一五”科技攻关项目(08010302149);教育部新世纪优秀人才基金项目(NCET-07-0251);安徽省第四批优秀青年科技基金项目(08040106803)

Optimization of Enzymatic Hydrolysis of Defatted Silkworm Pupa by Animal Proteases

ZHANG Hai-xiang1,WEI Zhao-jun1,*,ZHOU Le-chun1,LIAO Ai-mei1,2   

  1. 1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China;
    2. Department of Life Science, Hefei Teachers College, Hefei 230061, China
  • Received:2009-08-12 Online:2009-12-15 Published:2010-12-29
  • Contact: WEI Zhao-jun1,*, E-mail:zjwei@hfut.edu.cn

摘要:

采用响应曲面法对动物蛋白酶水解脱脂蚕蛹蛋白的工艺进行优化。在单因素考察的基础上,选定温度、pH 值、加酶量、液料比为试验因素,蚕蛹蛋白水解度为考察指标,分析结果表明,温度和加酶量的线性效应、温度和pH 值及液料比的平方效应、温度和pH 值的交互效应对蚕蛹蛋白水解度的影响达到极其显著水平。动物蛋白酶水解脱脂蚕蛹蛋白的最佳工艺为温度45℃、pH7.56、加酶量4.24%、液料比29.7、水解8h,在此条件下水解度为48.24%。

关键词: 蚕蛹蛋白, 水解度, 动物蛋白酶

Abstract:

Response surface methodology (RSM) was applied to optimize enzymatic hydrolysis of defatted silkworm pupa protein by commercial animal proteases. Effects of hydrolysis temperature, pH, protease amount and material-liquid ratio on the degree of hydrolysis (DH) were evaluated by single factor experiments. Results indicated that a linear relationship between hydrolysis temperature or protease amount and the degree of hydrolysis, a square effect of temperature, pH or material-liquid ratio on the degree of hydrolysis, cross-interaction of temperature and pH on the degree of hydrolysis were observed. The optimal hydrolysis condition for silkworm pupa protein was 45 ℃ of hydrolysis temperature, pH 7.56, 4.24% protease amount, and 1:29.7 of material-liquid ratio for 8 h of hydrolysis. The degree of hydrolysis of silkworm pupa protein was 48.24% under this optimal condition.

Key words: silkworm pupa protein, degree of hydrolysis, animal protease

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