食品科学 ›› 2018, Vol. 39 ›› Issue (7): 181-187.doi: 10.7506/spkx1002-6630-201807027

• 食品工程 • 上一篇    下一篇

微细化-蛋白酶解对蚕蛹蛋白营养价值和功能特性的影响

李少辉1,贾俊强1,2,桂仲争1,2,*   

  1. 1.江苏科技大学生物技术学院,江苏 镇江 212018;2.中国农业科学院蚕业研究所,江苏 镇江 212018
  • 出版日期:2018-04-15 发布日期:2018-04-17
  • 基金资助:
    公益性行业(农业)科研专项(201403064)

Effect of Micronization-Enzymatic Hydrolysis on Nutrition and Functional Properties of Silkworm (Bombyx mori) Pupa Protein

LI Shaohui1, JIA Junqiang1,2, GUI Zhongzheng1,2,*   

  1. 1. School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China; 2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China
  • Online:2018-04-15 Published:2018-04-17

摘要: 本实验采用磨球超微粉碎-蛋白酶解的方法改性处理蚕蛹蛋白,研究了改性处理后蚕蛹蛋白的营养价值和 功能特性。结果表明:微细化-酶解改性处理后蚕蛹蛋白的必需氨基酸/非必需氨基酸、必需氨基酸/总氨基酸均达到 联合国粮食及农业组织和世界卫生组织推荐模式标准,营养价值显著提升(P<0.05);改性处理后蚕蛹蛋白的水 解度提高了65.62%,溶解度、乳化性和起泡性分别提高了402%、187%和141%;巯基含量增加了57%,二硫键含量 降低了21%;二级结构无规卷曲含量由37.46%增加到91.33%;剂量为25 μg/mL的蚕蛹蛋白改性处理后,促小鼠脾细 胞增殖能力增加了100%,免疫能力极显著提高(P<0.01)。

关键词: 蚕蛹蛋白, 微细化, 蛋白酶解, 营养评价, 功能特性

Abstract: In this paper, the nutritional and functional properties of silkworm (Bombyx mori) pupa protein modified by micronization-enzymatic hydrolysis were studied. Results showed that the ratio of essential amino acids to non-essential amino acids and to total amino acids in the modified protein reached the Food and Agriculture Organization/World Health Organization (FAO/WHO) recommended pattern suggesting significantly improved nutritional value compared with the native protein (P < 0.05). The degree of hydrolysis was increased by 65.62%, and solubility, emulsifying capacity and foaming capacity by 402%, 187%, and 141%, respectively; the sulfhydryl group content was increased by 57% whereas disulfide content was decreased by 21%; the amount of random coil secondary structure was increased from 37.46% to 91.33% after the modification. The modified pupa protein at 25 μg/mL enhanced the proliferation of mouse splenocyte by 100%, suggesting a significant improvement of immunity (P < 0.01).

Key words: Bombyx mori pupa protein, micronization, enzymatic hydrolysis, nutritional value, functional properties

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