关键词: 蛹虫草, 发酵, 纤溶酶, 酶学性质

Abstract:

Fibrinolytic enzyme from Cordyceps militaris was extracted by ammonium sulfate precipitation and ion exchange chromatography and its enzymological properties wee also investigated. Results indicated that optimal pH and reaction temperature of this fibrinolytic enzyme were 8.0 and 37 ℃, respectively. This enzyme was stable at pH from 6.0 to 10.6, but sensitive to high temperature. Its activity could be inhibited by EDTA, which suggested this enzyme was a metalloproteinase. Moreover, its activity could be enhanced by Fe2+ and Ca2+ at a low concentration of 0.01 mol/L and inhibited by them at a high concentration of 0.1 mol/L. However, Na+ and NH4+ had little effect on the activity of this enzyme.

Key words: Cordyceps militaris, fermentation, fibrinolytic enzyme, enzymatic property