食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 288-291.doi: 10.7506/spkx1002-6630-200918065

• 分析检测 • 上一篇    下一篇

气相色谱分析测定高级烷醇的方法研究

马李一,张重权,王有琼,甘瑾,郑 华,郭元亨,赵虹,段琼芬*   

  1. 中国林业科学研究院资源昆虫研究所
  • 收稿日期:2009-05-26 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 段琼芬 E-mail:qiongfend@hotmail. com
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD06B07);国家林业局重点项目(2006-54)

Capillary GC Determination of Policosanols

MA Li-yi,ZHANG Zhong-quan,WANG You-qiong,GAN Jin,ZHENG Hua,GUO Yuan-heng, ZHAO Hong,DUAN Qiong-fen*   

  1. Research Institute of Resource Insects, Chinese Academy of Forestry, Kunming 650224, China
  • Received:2009-05-26 Online:2009-09-15 Published:2010-12-29
  • Contact: DUAN Qiong-fen E-mail:qiongfend@hotmail. com

摘要:

建立天然高级烷醇混合物含量分析的气相色谱方法。以三氯甲烷为溶剂,二十六烷烃为内标物,采用毛细管色谱柱:SE-30(30m × 0.32mm,0.25μm); 载气为N2,流量1.7ml/min;分流模式,分流比1:10;空气0.24MPa;氢气0.11MPa;进样口温度300℃;FID 检测器,检测温度300℃;柱温为290℃恒温模式;进样量0.2μl。二十六烷醇平均回收率高达98.85%(n=5),RSD 为1.02%;在8h 内样品和仪器性能稳定性好。该气相色谱检测分析方法重现性好、精密度高,完全可以满足高级烷醇混合物的分析测定。

关键词: 气相色谱, 高级烷醇, 内标, 峰面积

Abstract:

A new method was established for the determination of mixed policosanols by capillary gas chromatography (GC). Following extraction with chloroform, chromatographic separation and quantification were achieved on an SE-30 (30 m × 0.32 mm, 0.25 μm) capillary column using hexacosane as internal standard and N2 as carry gas with a flow rate of 1.7 ml/min in shunting model with a shunting ratio of 1:10 under the following conditions: air pressure 0.24 MPa, hydrogen pressure 0.11 MPa, injection port temperature 300 ℃, detection temperature of FID detector 300 ℃, constant column temperature 290 ℃, and injection volume 0.2 μl. The average spiked recovery of hexacosanol was 98.85% (n = 5), with a relative standard deviation (RSD) of 1.02%. Within 8 h, both test sample, a mixture of 4 policosanols and instrument performance showed good stability. This method has good repeatability and high precision, and therefore can be used to determinate policosanols.

Key words: gas chromatography (GC), policosanol, internal standard, peak area

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