食品科学 ›› 2009, Vol. 30 ›› Issue (13 ): 179-182.doi: 10.7506/spkx1002-6630-200913042

• 生物工程 • 上一篇    下一篇

一株蓝麻黄内生细菌的分离鉴定和抑菌活性研究

古力山·买买提,赵国玉,吾甫尔·米吉提*   

  1. 新疆大学生命科学与技术学院
  • 收稿日期:2009-01-14 出版日期:2009-07-01 发布日期:2010-12-29
  • 通讯作者: 吾甫尔·米吉提* E-mail:gmijit2001@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目(30560004)

Isolation, Identification and Antibacterial Activities of Endophytic Bacterial Strain XJ-EG-GA-13 from E.glauca Stems

GULISHAN·Maimaiti,ZHAO Guo-yu,GOPUR·Mijit*   

  1. ( College of Life Science and Technology, Xinjiang University, Urumqi 830046, China)
  • Received:2009-01-14 Online:2009-07-01 Published:2010-12-29
  • Contact: GOPUR·Mijit* E-mail:gmijit2001@yahoo.com.cn

摘要:

为筛选具有拮抗活性的植物内生菌,以新疆有毒植物蓝麻黄(E.glauca)为材料,采用研磨法和琼脂扩散法从中分离并筛选出一株对多种植物致病菌具有较强抗性的内生细菌XJEG-GB-13。对其生物学特性进行初步研究,研究表明,最适生长温度为37℃,最适生长pH 值为6.0。根据其形态特征、生理生化检测、16S rDNA 测序,将其鉴定为枯草芽孢杆菌的一个亚种Bacillus subtilis subsp. subtilis。XJEG-GB-13 的获得可为进一步研究高效拮抗菌的拮抗机制提供实验材料。

关键词: 蓝麻黄, 内生菌, 拮抗作用, 分离, 鉴定

Abstract:

A novel endophytic bacterial strain XJ-EG-GB-13 against several species of plant pathogenic bacteria was isolated and identified from E.glauca stems by grinding method and agar diffusion test. The biological properties of the XJ-EG-GB-13 were also under this investigation. The results showed that the optimal growth temperature and pH value for the XJ-EG-GB- 13 were 37 ℃ and 6, respectively. According to morphological characteristics, physiological and biochemical properties and 16 S rDNA sequencing, the XJ-EG-GB-13 had 99.791% homology to Bacillus.subtilis subsp.subtilis. The results suggested that the XJ-EG-GB-13 is a potential and promising bacterium for studies of the mechanism of antagonist.

Key words: E.glauca, endophyte, antagonistic action, isolation, identification

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