食品科学 ›› 2021, Vol. 42 ›› Issue (17): 193-200.doi: 10.7506/spkx1002-6630-20200820-278

• 包装贮运 • 上一篇    下一篇

解淀粉芽孢杆菌NCPSJ7对采后脐橙绿霉病的防治作用及机制

阎然,傅茂润,陈蕾蕾,李有媛,徐敏慧,何志平,周庆新   

  1. (1.齐鲁工业大学(山东省科学院)食品科学与工程学院,山东 济南 250353;2.山东省农业科学院农产品研究所,山东 济南 250100;3.诸城一中慈海学校,山东 诸城 262201;4.浙江农林大学农业与食品科学学院,浙江 临安 311300)
  • 发布日期:2021-09-29
  • 基金资助:
    国家自然科学基金面上项目(31871854);“十三五”国家重点研发计划重点专项(2017YFD0401304)

Effect and Mechanism of Bacillus amyloliquefaciens NCPSJ7 against Green Mold on Navel Oranges

YAN Ran, FU Maorun, CHEN Leilei, LI Youyuan, XU Minhui, HE Zhiping, ZHOU Qingxin   

  1. (1. School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China;2. Institute of Agro-Food Sciences and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 3. Zhucheng Yizhong Cihai School, Zhucheng 262201, China; 4. College of Agriculture and Food Science, Zhejiang A & F University, Lin’an 311300, China)
  • Published:2021-09-29

摘要: 为了阐明解淀粉芽孢杆菌NCPSJ7对采后脐橙绿霉病的防治作用以及潜在抑菌机理,以NCPSJ7发酵原液为实验材料,采用牛津杯以及果实打孔接种的方法,测定脐橙果实在贮藏过程中发病率、抗病性相关酶活力、抗氧化能力等指标。体外实验结果表明,高浓度的NCPSJ7显著抑制指状青霉(Penicillium digitatum)的生长(P<0.05)。当NCPSJ7浓度达到1×108 CFU/mL时,其抑菌圈的直径为3.50 cm,是1×105 CFU/mL处理组(1.75 cm)的2 倍。体内实验结果表明,NCPSJ7在接种24 h时可定植到脐橙表皮伤口处,通过抢占空间和营养来抑制指状青霉的生长。在室温(25 ℃)条件下贮藏,与对照组和指状青霉单一处理组相比,NCPSJ7和指状青霉复合处理明显降低了脐橙的发病率和病斑直径,提高了果实中多酚氧化酶、过氧化物酶、几丁质酶和β-1,3-葡聚糖酶活力,促进果实的总酚含量、总黄酮含量、1,1-二苯基-2-三硝基苯肼自由基清能力和还原力的增加。综上所述,解淀粉芽孢杆菌NCPSJ7可作为一种有效防治采后脐橙绿霉病的微生物拮抗剂。

关键词: 脐橙;拮抗作用;指状青霉;抗氧化能力;作用机制

Abstract: The objective of this study was to evaluate the effect of Bacillus amyloliquefaciens NCPSJ7 against green molds on postharvest navel oranges and the underlying mechanism. The antifungal effect of cultures of NCPSJ7 was determined by the Oxford cup method and disease incidence, disease resistance-related enzymes activities and antioxidant capacity in oranges artificially inoculated, treated with the fermentation broth and inoculated with Penicillium digitatum were determined. The results indicated that a high NCPSJ7 concentration significantly inhibited the growth of P. digitatum on navel oranges in vitro (P < 0.05). The diameter of inhibition zone of 1 × 108 CFU/mL NCPSJ7 was 3.50 cm, which was twice higher than that observed at 1 × 105 CFU/mL (1.75 cm). In vivo tests showed that the antagonist NCPSJ7 could colonize rapidly the wounds in oranges within 24 h after inoculation for space and nutrient competition to inhibit the growth of P. digitatum. Moreover, compared with single P. digitatum and the control group, NCPSJ7 combined with P. digitatum significantly decreased disease incidence and lesion diameter, increased the activities of polyphenol oxidase (PPO), peroxidase (POD), chitinase (CHI) and β-1,3 glucanase (GLU), and promoted the increase in reducing power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity as well as total phenol and total flavonoid contents. These observations indicated that B. amyloliquefaciens NCPSJ7 could be used as a biocontrol agent against P. digitatum on postharvest oranges.

Key words: navel oranges; antagonism; Penicillium digitatum; antioxidant capacity; action mechanisms

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