食品科学 ›› 2008, Vol. 29 ›› Issue (1): 91-94.

• 基础研究 • 上一篇    下一篇

五味子多糖的分离纯化及清除自由基研究

 孟宪军, 秦琴, 高晓旭   

  1. 沈阳农业大学食品学院; 沈阳农业大学食品学院 辽宁沈阳110161; 辽宁沈阳110161;
  • 出版日期:2008-01-15 发布日期:2011-07-28

Study on Extraction, Purification and Scavenging Free Radical of Polysaccharide SCP-B II from Schisandra chinensis

 MENG  Xian-Jun, QIN  Qin, GAO  Xiao-Xu   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110161, China
  • Online:2008-01-15 Published:2011-07-28

摘要: 应用水提醇沉以及多种方法除蛋白,从五味子果实中获得一种粗多糖SCP-BII。SCP-BII经DEAE-Cellu-lose52和Sephadex G-200柱层析后得到单一组分SCP-BII。应用化学发光法对SCP-BII的清除·OH和O2·的能力进行了研究,结果表明:SCP-BII对·OH和O2·均具有明显的清除能力。

关键词: 五味子多糖, 分离纯化, 清除自由基

Abstract:  A polysaccharide SCP-B II was obtained from Schisandra chinensis Turcz.Bail.fruits by method of water extraction and ethanol precipitation for protein removal. After DEAE-Cellulose 52 and Sephadex G-200 column chromatography, single component SCP-B II was got. The scavenging ability of ·OH and O·2 of SCP-B II were assayed by chemiluminescence. The results showed that SCP-B II have evident abilities of scavenging ·OH and O·2.

Key words: Schisandra chinensis polysaccharides, purification, scavenging free radical