食品科学 ›› 2008, Vol. 29 ›› Issue (4): 114-116.

• 基础研究 • 上一篇    下一篇

壳寡糖对肠道致病菌抑制作用的研究

 曹维强, 王静, 佘永新, 赵海田, 张英春, 杨鑫, 张华   

  1. 中国农业科学院农业质量标准与检测技术研究所; 哈尔滨工业大学食品科学与遗传工程研究院; 哈尔滨工业大学食品科学与遗传工程研究院; 惠州出入境检验检疫局;
  • 出版日期:2008-04-15 发布日期:2011-08-24

Study on Inhibitive Effect of Chitooligosaccharides on Intestinal Causative Microorganism

 CAO  Wei-Qiang, WANG  Jing, SHE  Yong-Xin, ZHAO  Hai-Tian, ZHANG  Ying-Chun, YANG  Xin, ZHANG  Hua   

  1. 1. Institute of Quality Standard and Testing Technology of Agricultral Product, The Chinese Academy of Agricultural Sciences;2. Huizhou Exit-Entry Inspection and Quarantine Bureau; 3. College of Food Science and Genetic Engineering, Harbin Institute of Technology
  • Online:2008-04-15 Published:2011-08-24

摘要: 采用过氧化氢氧化法制备水溶性壳寡糖,采用QTRAPLC/MS/MSSystem测定壳寡糖的平均分子量,并对4种肠道致病菌进行了抗菌实验,其抑菌率明显超过对照组,壳寡糖对大肠埃希氏菌和金黄色葡萄球菌的最低抑制浓度为0.3%,对副伤寒甲沙门氏菌、宋内志贺菌的最低抑制浓度为0.1%,随着壳寡糖质量浓度增加,抑菌作用逐渐变强。结果证明,壳寡糖对肠道致病菌具有良好的抑制作用。

关键词: 壳寡糖, 肠道致病菌, 抗菌作用

Abstract: Chitooligosaccharides were prepared by hydrogen peroxide method, with Q TRAP LC/MS/MS System to determine chitooligosaccharides’ average molecular weight and on the inhibitive effects of chitooligosaccharides on four species intestinal causative microorganism were studied. The inhibitive function is obvious compared with the blank. The results showed that the lowest concentration of chitooligosaccharides inhibiting Esaherichiacoli and Staphylococcusaureus is 0.3%, and the lowest concentration of chitooligosaccharides inhibiting S.paratyphi A and Shigella-sonnei is 0.1%. With the increasing of concentration of chitooligosaccharides, the inhibitive function is enhanced. The results showed that the chitooligosaccharides has good inhibitive effect on intestinal causative microorganism.

Key words: chitooligosaccharides, intestinal causat ive microorganism, antimicrobial activity