食品科学 ›› 2008, Vol. 29 ›› Issue (8): 433-436.

• 生物工程 • 上一篇    下一篇

不同碳源对黑曲霉产糖化酶活力的影响

孙俊良, 李新华, 梁新红, 赵瑞香   

  1. 沈阳农业大学食品学院; 河南科技学院食品学院
  • 出版日期:2008-08-15 发布日期:2011-08-26

Effects of Different Carbon Sources on Activity of Glucoamylase Produced by Aspergillus niger

 SUN  Jun-Liang, LI  Xin-Hua, LIANG  Xin-Hong, ZHAO  Rui-Xiang   

  1. 1.College of Food Science,Shenyang Agricultural University,Shenyang 110161,China;2.School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 碳源对黑曲霉产糖化酶活力有很大影响。研究可溶性淀粉、葡萄糖、蔗糖、麦芽糖、乳糖和β-环糊精为碳源时,分别对黑曲霉产糖化酶活力的影响。结果表明,可溶性淀粉为碳源时酶活力最高,最高可达350.34±14.06U/ml;其次是葡萄糖;蔗糖和麦芽糖为碳源时酶活力没有显著性差异;乳糖为碳源时酶活力低于蔗糖和麦芽糖;β-环糊精的酶活力最低。不同碳源发酵培养基中生物量研究表明,黑曲霉能充分利用可溶性淀粉、葡萄糖、蔗糖和麦芽糖,对乳糖的利用较差,几乎不能利用β-环糊精。

关键词: 碳源, 黑曲霉, 糖化酶

Abstract: Choosing soluble starch,glucose,sucrose,maltose,lactose and β-cyclodextrin as carbon sources of A. niger to produce glucoamulase,their effects on the activity of glucoamulase were studied. The results showed that the glucoamylase activity is the highest,reaching 350.34±14.06 U/ml,when soluble starch was used as carbon source,followed by glucose,sucrose and maltose in turn(no significant difference at 0.05 probability level among them) . The glucoamylase activity by lactose is less than that by sucrose or maltose. Almost no glucoamylase is produced when β-cyclodextrin is used as carbon source. The results of mycelia biomass of A. niger showed that A. niger can assimilate starch,glucose,sucrose and maltose apart from β-cyclodextrin.

Key words: carbon source, Aspergillus niger, glucoamylase