食品科学 ›› 2008, Vol. 29 ›› Issue (3): 307-309.
• 生物工程 • 上一篇 下一篇
王年久, 马爱民
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WANG Nian-Jiu, MA Ai-Min
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摘要: 本实验采用一步法、直接法和沉淀法分别对平菇差异显示PCR银染产物中粗、细两种特异条带进行回收,并进行再扩增效果比较。结果表明,三种特异条带的回收方法中,直接法操作简单、快速可行,且再扩增效果优于一步法和沉淀法,可有效应用于平菇mRNA差异显示实验。
关键词: 平菇, 差异显示, 一步法, 直接法, 沉淀法
Abstract: Three methods including one-step method, direct method and deposited method were used to recover the thick and thin bands from the silver stained differential display PCR products in Pleurotus ostreatus and their re-amplification effects were evaluated. The results showed that the direct method is a rapid, simple and reliable method among the three different recovering methods. And its re-amplification effect is better than that of the one-step method and the deposited method. So it can be efficiently applied in mRNA differential display PCR experiment of Pleurotus ostreatus.
Key words: Pleurotus ostreatus, differential display, one-step method, direct method, deposited method
王年久, 马爱民. 平菇差异显示PCR产物特异条带的回收方法及再扩增效果[J]. 食品科学, 2008, 29(3): 307-309.
WANG Nian-Jiu, MA Ai-Min. Recovering Methods and Re-amplified Effects of Special Bands in Differential Display PCR Products in Pleurotus ostreatus[J]. FOOD SCIENCE, 2008, 29(3): 307-309.
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