食品科学 ›› 2007, Vol. 28 ›› Issue (7): 158-161.

• 工艺技术 • 上一篇    下一篇

小血藤总黄酮的提取研究

 赵虹桥, 唐克华, 陈功锡, 龚桂生   

  1. 吉首大学城乡资源与规划学院; 吉首大学城乡资源与规划学院 湖南张家界427000; 湖南张家界427000;
  • 出版日期:2007-07-15 发布日期:2011-10-24

Comparative Study on Different Extraction Methods of Total Flavonoids and Contents Determination from Roots, Stems and Leaves of Schisandra Propinqua

 ZHAO  Hong-Qiao, TANG  Ke-Hua, CHEN  Gong-Xi, GONG  Gui-Sheng   

  1. College of Resources and Planning Science, Jishou University, Zhangjiajie 427000, China
  • Online:2007-07-15 Published:2011-10-24

摘要: 运用索式回流提取法、碱提酸沉法、水浴浸提法提取小血藤根、茎、叶中总黄酮,以硝酸铝显色法测定其总黄酮的含量。进一步以正交试验法研究适宜小血藤根的索氏法提取条件,结果表明:小血藤根、茎、叶不同部位的总黄酮提取,均以索式回流提取法为最好;以石油醚在50℃下回流2h去脂多次,85%乙醇按料液比为1:15于70℃下提取3h,测得小血藤根、茎、叶中总黄酮含量分别为1.67%、0.94%、2.35%。

关键词: 小血藤, 总黄酮, 提取, 测定, 含量

Abstract: Soxlet extraction, alkali extraction and water bathe extraction methods were employed to extract the total flavonoids from roots, stems, leaves of Schisandra Propinqua, the contents of total flavonoids and were determined by means of aluminium nitrate colouration approach. The experiment results indicated that the optimum extraction method of the total flavonoids from the different parts in Schisandra Propinqua is Soxlet extraction. By extracting lipid with benzine in 2 h under 50 ℃ and then extracting total flavonoids by 85% ethanol (material ratio 1:15) in 3 h under 70 ℃, the total flavonoids content of the roots, stems and leaves in Schisandra Propinqua are 1.67%, 0.94% and 2.35% respectively.

Key words: Schisandra Propinqua, total flavonoids, extraction, determination, content