食品科学 ›› 2006, Vol. 27 ›› Issue (2): 232-234.

• 分析检测 • 上一篇    下一篇

RP—HPLC法测定保健饮料中羟基红花黄色素A的含量

 刘红, 郁晓艺, 李炳奇, 田丽萍, 汪河滨   

  1. 石河子大学化学系; 石河子大学生命科学学院
  • 出版日期:2006-02-15 发布日期:2011-09-05

Detection of Hydroxy Safflor Yellow A in Health Foods by RP-HPLC

 LIU  Hong, YU  Xiao-Yi, LI  Bing-Qi, TIAN  Li-Ping, WANG  He-Bin   

  1. 1.Department of Chemistry, Shihezi University, Shihezi 832000, China ; 2.College of Life Science, Shihezi University, Shihezi 832000, China.
  • Online:2006-02-15 Published:2011-09-05

摘要: 采用反相液相色谱法测定保健食品中羟基红花黄色素A的含量,使用Kromasil-C18柱,甲醇-0.03mol/L醋酸溶液(V:V=22:78)为流动相,流速1.0ml/min,柱温20℃,波长403nm。本法快捷、简便精密、分离效果好、平均回收率98.5%,适用于保健食品中羟基红花黄色素A的测定。

关键词: 羟基红花黄色素A, 反相液相色谱

Abstract:  To determine the content of hydroxysafflor yellow A in the health foods by RP-HPLC, the hydroxysafflor yellow A could be separated on Kromasil-C 18 with nonlinearity gradient elution of CH 3OH-HAc(22:78) at a flow -rate of 1.0ml/min and also assayed at UV403nm, with the column temperature 25 ℃. The average recovery of sample was 98.5% 。This method was simple, rapid, accurate and reiable. It is suitable for the determination of HSYA concentration in health foods.

Key words: hydroxysafflor yellow A, RP-HPLC