食品科学 ›› 2006, Vol. 27 ›› Issue (2): 230-232.

• 分析检测 • 上一篇    下一篇

双波长分光光度法测定黑玉米花粉中总黄酮的含量

 张兰杰, 辛广, 张维华   

  1.  鞍山师范学院化学系
  • 出版日期:2006-02-15 发布日期:2011-09-05

Assaying Total Flavonoids in Black Corn Pollen by Double-wavelength Spectrophotometry

 ZHANG  Lan-Jie, XIN  Guang, ZHANG  Wei-Hua   

  1. Department of Chemistry, Anshan Normal University, Anshan 114005, China
  • Online:2006-02-15 Published:2011-09-05

摘要: 介绍了一种双波长分光光度法,应用于测定黑玉米花粉中的总黄酮含量,测定波长510nm,参比波长584nm,平均回收率98.14%,RSD=1.24%(n=5),结果表明,黑玉米花粉中总黄酮平均含量为3.53%,该方法稳定可靠,重现性好。

关键词: 黑玉米花粉, 总黄酮, 双波长分光光度法

Abstract:  In this paper, a double-wavelength spectrophotometry was applied to assay the total flavonoids in black corn pollen. The detective wavelength was 510 nm ,while the reference one was 584 nm, and the recovery rate was 98.14%, with RSD=1.24% (n=5).The results indicated that the average total flavonoids content in black corn pollen was 3.53%, and the method was stable, reliable and accurate in the assay process.

Key words: black corn pollen, total flavonoids, double-wavelength spectrophotometry