食品科学 ›› 2019, Vol. 40 ›› Issue (3): 42-47.doi: 10.7506/spkx1002-6630-20180107-069

• 基础研究 • 上一篇    下一篇

木槿花生物活性物质及其抗氧化活性分析

黄采姣1,2,李安平1,2,*,李建周1,王晓红1   

  1. (1.中南林业科技大学食品科学与工程学院,湖南?长沙 410004;2.特医食品加工湖南省重点实验室,湖南?长沙 410004)
  • 出版日期:2019-02-15 发布日期:2019-03-05
  • 基金资助:
    国家林业局948项目(20150417);湖南省科技创新平台与人才计划项目(2017TP1021)

Bioactive Components of Hibiscus Flower and Their Antioxidant Activity

HUANG Caijiao1,2, LI Anping1,2,*, LI Jianzhou1, WANG Xiaohong1   

  1. (1. College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China; 2. Hunan Key Laboratory of Processed Food for Special Medical Purpose, Changsha 410004, China)
  • Online:2019-02-15 Published:2019-03-05

摘要: 研究5?种木槿花卉及其不同极性溶剂萃取物的生物活性物质含量和抗氧化活性,分析它们之间的相关性,并采用高效液相色谱-串联质谱鉴定其中的活性物质成分,以期为木槿深加工及其品种选育提供理论依据。5?个品种木槿花的总酚和总黄酮含量从高到低依次为木槿原种、粉紫重瓣木槿花、薰衣草薄绸木槿花、紫玉和雅致木槿,各品种木槿花的抗氧化活性排序与其总酚和总黄酮含量排序基本一致,其中木槿原种的总酚和总黄酮含量最高,分别达到了17.33?mg/g和12.68?mg/g,且抗氧化活性最强,其总还原能力、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、2,2’-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)自由基清除能力分别为1.58?mmol/g和6.82、17.96?mg/g。木槿花的抗氧化活性与其总酚和总黄酮含量间有极显著的相关性(P<0.01),与花青素含量相关性不显著(P>0.05)。木槿原种不同极性溶剂萃取物均具有一定的抗氧化活性,其中正丁醇的总还原能力(6.16?mmol/g)最高,且对DPPH自由基(15.50?mg/g)和ABTS自由基(40.71?mg/g)清除效果最好。从木槿原种正丁醇萃取物中初步鉴定出8?种抗氧化物质,分别为山柰酚-O-六碳糖-C-六碳糖苷、芹菜素、山柰酚-O-六碳糖-C-六碳糖苷同分异构体、芹菜素-C-二糖苷、芹菜素-葡萄糖芹糖苷、山柰酚-3-O-芸香糖苷、芹菜素-7-O-芸香糖苷、矢车菊素-3-丙二酰葡萄糖苷。

关键词: 木槿花, 总酚, 总黄酮, 抗氧化活性

Abstract: This study aimed to investigate and correlate the bioactive components and antioxidant activity of flowers from five varieties of Hibiscus syriacus and different polar solvent extracts from Hibiscus syriacus L. for the purpose of providing a theoretical basis for deep processing and breed of hibiscus flower. The bioactive components were identified by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results showed that the contents of total polyphenols and total flavonoids in five varieties of Hibiscus syriacus were consistently in the decreasing order of Hibiscus syriacus L., Hibiscus syriacus f. paeoniflorus, Hibiscus syriacus cv. Lavandula Chiffon, Hibiscus syriacus cv. Shigyoku and Hibiscus syriacus f. elegantissimus. A similar order was observed in the antioxidant activity. The contents of polyphenols and total flavonoids in the flower of Hibiscus syriacus L. were the highest, which were 17.33 and 12.68?mg/g, respectively, and it had the strongest antioxidant activity with ferric ion reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt? (ABTS) radical scavenging capacity of 1.58?mmol/g, 6.82 and 17.96?mg/g, respectively. There was a significant correlation between the antioxidant activity and the contents of total polyphenols and total flavonoids (P < 0.01), but the correlation with anthocyanin content was not significant (P > 0.05). All solvent extracts from Hibiscus syriacus L. had antioxidant activity. The highest total reducing power (6.16 mmol/g) was observed in the n-butanol extract, accompanied by the strongest DPPH radical (15.50?mg/g) and ABTS radical scavenging capacity (40.71?mg/g). Eight compounds from the n-butanol extract were identified including kaempferol-O-hexose-C-glucoside, apigenin, kaempferol-O-hexose-C-hexose glycoside isomers, apigenin-C-diglycoside, apigenin-glycoside glucoside, behenylphenol-3-O-rutinoside, apigenin-7-O-rutinoside, and cyanidin-3-malonyl glucoside.

Key words: hibiscus flower, total polyphenols, total flavonoids, antioxidant activity

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