澳洲坚果青皮不同极性溶剂分步提取物功能成分与抗氧化活性及其相关性分析

doi:10.7506/spkx1002-6630-20200310-154

食品科学 ›› 2021, Vol. 42 ›› Issue (7): 74-82.doi: 10.7506/spkx1002-6630-20200310-154

澳洲坚果青皮不同极性溶剂分步提取物功能成分与抗氧化活性及其相关性分析

郭刚军，胡小静，付镓榕，马尚玄，徐荣，黄克昌，彭志东，贺熙勇，邹建云

（1.云南省热带作物科学研究所，云南景洪 666100；2.文山学院化学与工程学院，云南文山 663000）

出版日期:2021-04-15 发布日期:2021-05-17
基金资助:
云南省热带作物科技创新体系建设专项（RF2020-14；RF2019-11；RF2018-10）；云南省热带作物科学研究所青年成长基金项目（QNCZ2020-5）

Determination and Correlation Analysis of Functional Components and Antioxidant Activity of Successive Solvent Extracts from Macadamia Green Husk

GUO Gangjun, HU Xiaojing, FU Jiarong, MA Shangxuan, XU Rong, HUANG Kechang, PENG Zhidong, HE Xiyong, ZOU Jianyun

(1. Yunnan Institute of Tropical Crops, Jinghong 666100, China; 2. School of Chemistry and Engineering, Wenshan University, Wenshan 663000, China)

Online:2021-04-15 Published:2021-05-17

摘要/Abstract

摘要： 采用80%（体积分数，下同）乙醇超声提取澳洲坚果青皮，依次用石油醚、氯仿、乙酸乙酯、正丁醇4 种不同极性溶剂对其提取物进行分步萃取，剩余为水溶解物，分别得到80%乙醇提取物、石油醚提取物、氯仿提取物、乙酸乙酯提取物、正丁醇提取物与水溶解物6 种溶剂提取物，测定其总酚、黄酮、多糖等主要功能成分质量分数与抗氧化活性，并分析其相关性。结果表明：总酚和黄酮质量分数在澳洲坚果青皮乙酸乙酯提取物中均最高，分别为（40.36±0.48）%与（41.68±0.93）%，多糖质量分数在正丁醇提取物中最高，为（22.08±2.09）%。澳洲坚果青皮不同极性溶剂分步提取物具有较强的2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸（2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)，ABTS）阳离子自由基清除能力与较高的还原力，对1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基与超氧阴离子自由基拥有一定的清除能力。其中，乙酸乙酯提取物对DPPH自由基、ABTS阳离子自由基的清除能力最强，还原力最高，其半抑制浓度（half maximal inhibitory concentration，IC50）分别为0.67、0.05、0.09 mg/mL。正丁醇提取物对超氧阴离子自由基的清除能力最强，其IC50为0.08 mg/mL，相同质量浓度下优于芦丁。通过对各指标进行皮尔逊相关性分析得出，澳洲坚果青皮不同极性溶剂分步提取物对DPPH自由基清除能力、ABTS阳离子自由基清除能力与还原力均与其总酚、黄酮、多糖质量分数呈极显著正相关（P＜0.01），对超氧阴离子自由基的清除能力与其多糖质量分数呈极显著正相关（P＜0.01）。应用多元回归分析建立了澳洲坚果青皮不同极性溶剂分步提取物DPPH自由基清除能力（Y1）、超氧阴离子自由基清除能力（Y2）、ABTS阳离子自由基清除清除能力（Y3）、还原力（Y4）与其功能成分（总酚（X1）、黄酮（X2）、多糖（X3）与皂苷及其他成分（X4））质量分数之间的线性回归方程，分别为Y1＝7.634 4－0.071 0X1＋0.170 2X2＋0.227 6X3－0.013 3X4、Y2＝29.024 5－0.405 0X1＋0.320 0X2＋0.597 2X3、Y3＝40.305 6＋0.188 8X1＋0.030 4X4与Y4＝0.298 2＋0.004 5X2＋0.006 0X3。结论：研究为开发利用澳洲坚果青皮资源提供一定的技术依据。

关键词: 澳洲坚果青皮；不同极性溶剂；分步提取物；功能成分；抗氧化活性；相关性

Abstract: The contents of total phenols, total flavonoids and polysaccharides and the in vitro antioxidant activities of petroleum ether (PE), chloroform (CE), ethyl acetate (EAE), n-butanol (NBE) and water soluble extracts obtained from successive fractionation of the 80% ethanol extract (EE) of macadamia green husk were evaluated and the correlation between the bioactive components and the antioxidant activities was analyzed. The results showed that the contents of total phenols and flavonoids in EAE were the highest among all samples, (40.36 ± 0.48)% and (41.68 ± 0.93)%, respectively, and the content of polysaccharides in NBE was the highest, (22.08 ± 2.09)%. EE and fractions thereof all had strong scavenging capacity against 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radicals and reducing power, and also had certain scavenging capacity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion radicals. EAE had?the strongest scavenging capacity against DPPH and ABTS cation radicals and the highest reducing power with half maximal inhibitory concentration (IC50) of 0.67, 0.05 and 0.09 mg/mL, respectively. NBE had the strongest scavenging capacity against superoxide anion radicals with IC50 of 0.08 mg/mL, which was better than rutin at the same concentration. Pearson correlation analysis showed that for all samples, the scavenging effect on DPPH and ABTS cation radicals and reducing power were significantly positively correlated with the contents of total phenols, flavonoids and polysaccharides (P < 0.01), and the scavenging effect on superoxide anion radicals was significantly positively correlated with the content of polysaccharides (P < 0.01). The linear regression equations?between antioxidant properties and the contents of total phenols (X1), flavonoids (X2), polysaccharides (X3) and Saponins and other components (X4) were established as follows: Y1 = 7.634 4 ? 0.071 0X1 + 0.170 2X2 + 0.227 6X3 ? 0.013 3X4 for DPPH scavenging capacity, Y2 = 29.024 5 ? 0.405 0X1 + 0.320 0X2 + 0.597 2X3 for superoxide anion radical scavenging capacity, Y3 = 40.305 6 + 0.188 8X1 + 0.030 4X4 for ABTS cation radical scavenging capacity, and Y4 = 0.298 2 + 0.004 5X2 + 0.006 0X3 for reducing power, respectively. Conclusion: This study provides a certain technical basis for the development and utilization of macadamia green husk.

Key words: macadamia green husk; different polar solvents; successive fractionation; functional components; antioxidant activity; correlation

中图分类号:

TS201.4

郭刚军，胡小静，付镓榕，马尚玄，徐荣，黄克昌，彭志东，贺熙勇，邹建云. 澳洲坚果青皮不同极性溶剂分步提取物功能成分与抗氧化活性及其相关性分析[J]. 食品科学, 2021, 42(7): 74-82.

GUO Gangjun, HU Xiaojing, FU Jiarong, MA Shangxuan, XU Rong, HUANG Kechang, PENG Zhidong, HE Xiyong, ZOU Jianyun. Determination and Correlation Analysis of Functional Components and Antioxidant Activity of Successive Solvent Extracts from Macadamia Green Husk[J]. FOOD SCIENCE, 2021, 42(7): 74-82.

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澳洲坚果青皮不同极性溶剂分步提取物功能成分与抗氧化活性及其相关性分析

Determination and Correlation Analysis of Functional Components and Antioxidant Activity of Successive Solvent Extracts from Macadamia Green Husk

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