食品科学 ›› 2021, Vol. 42 ›› Issue (15): 143-149.doi: 10.7506/spkx1002-6630-20200724-330

• 营养卫生 • 上一篇    下一篇

羧甲基普鲁兰多糖螯合钙的制备及其功效评价

李海鹰,杨祥禾,魏珍珍,贾蓓,杨文智   

  1. (河北大学药学院,河北省药物质量分析控制重点实验室,河北 保定 071002)
  • 出版日期:2021-08-15 发布日期:2021-08-27
  • 基金资助:
    河北省自然科学基金面上项目(C2021201026;H2018201045);河北省高等学校科学技术研究项目(ZD2018054)

Preparation and Evaluation of Carboxymethyl Pullulan-Calcium Complex in Vivo

LI Haiying, YANG Xianghe, WEI Zhenzhen, JIA Bei, YANG Wenzhi   

  1. (Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China)
  • Online:2021-08-15 Published:2021-08-27

摘要: 为研究羧甲基普鲁兰多糖螯合钙的功效,以天然普鲁兰多糖为原料,通过羧甲基化反应合成水溶性羧甲基普鲁兰多糖(carboxymethyl pullulan,CMP),随后利用CMP与钙溶液反应制备CMP-Ca(II)配合物,通过紫外光谱、傅里叶变换红外光谱和扫描电子显微镜对其结构进行表征,以滴定法测定的CMP-Ca(II)中钙离子质量分数为指标,采用单因素试验和响应面试验优化CMP-Ca(II)制备条件,最后建立骨质疏松Balb/C小鼠模型,利用CMP-Ca(II)干预,通过测定骨质量、骨密度和骨硬度等指标研究CMP-Ca(II)对骨质疏松小鼠的补钙效果。紫外光谱和傅里叶变换红外光谱分析结果表明,CMP中羧基和羟基与钙离子发生螯合。经单因素试验和响应面试验优化得到的最佳CMP-Ca(II)制备条件为Ca2+与CMP质量比2∶1、反应液pH 10、温度50 ℃;在此条件下,可制得钙离子质量分数为10.3%的CMP-Ca(II)。骨质疏松小鼠经CMP-Ca(II)干预后,骨质量、骨密度和骨硬度提高,血清碱性磷酸酶活力降低,表明CMP-Ca(II)能够调节骨质疏松小鼠的钙吸收能力,缓解骨质疏松,改善小鼠骨骼状况。本实验可为合成和开发新型CMP-Ca(II)补钙制剂提供参考。

关键词: 普鲁兰多糖;羧甲基普鲁兰多糖螯合钙;补钙制剂;骨质疏松症

Abstract: Carboxymethyl pullulan-calcium chelate [CMP-Ca (II)] was synthesized by the reaction between water-soluble carboxymethyl pullulan (CMP), prepared from pullulan, and Ca2+ and was structurally characterized by ultraviolet (UV) spectroscopy, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Based on the calcium content in CMP-Ca (II) determined by titration, one-factor-at-a-time method and response surface methodology with Box-Behnken Design were used jointly to determine the optimal preparation conditions for CMP-Ca (II). Finally, a Balb/C mouse model of osteoporosis was created to evaluate the efficacy of CMP-Ca (II) as a calcium supplement by measuring bone mass, mineral density and hardness. The results of UV and FTIR showed that carboxyl and hydroxyl groups in CMP chelated with calcium ions. The optimal conditions for preparing CMP-Ca (II) were as follows: Ca2+/CMP mass ratio 2:1, pH 10 and 50 ℃, and the calcium content of CMP-Ca (II) prepared under the optimized conditions was 10.3%. The animal experiment results showed that CMP-Ca (II) was able to improve the bone mass, mineral density and hardness of mice suffering from osteoporosis and reduce serum alkaline phosphatase (ALP) activity, indicating that CMP-Ca (II) could regulate calcium absorption in mice with osteoporosis, relieve osteoporosis and improve bone status. The method for preparing CMP-Ca (II) presented in this study proved to be simple and convenient and could provide a strategy for the development of a new calcium supplement.

Key words: pullulan; carboxymethyl pullulan-calcium complex; calcium supplement; osteoporosis

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