食品科学 ›› 2005, Vol. 26 ›› Issue (1): 121-124.

• 工艺技术 • 上一篇    下一篇

双水相系统中酶法水解明胶制取氨基酸的研究

 张刚, 黄登禹, 张丛越   

  1. 天津市工业微生物研究所; 天津市工业微生物研究所
  • 出版日期:2005-01-15 发布日期:2011-09-19

Study on Aqueous Two-phase Systems to Produce Amino Acids Through Gelatin Hydrolysis

 ZHANG  Gang, HUANG  Deng-Yu, ZHANG  Cong-Yue   

  1. Tianjin Research Institute of Industrial Microbiology
  • Online:2005-01-15 Published:2011-09-19

摘要: 在PEG—盐双水相系统中,进行了蛋白酶催化明胶水解制取混合氨基酸的研究。双水相系统组成为20%PEG1540、10%磷酸钾盐、pH7.5时,加入3%碱性蛋白酶发酵液和35%明胶,45℃下水解10h,转化率达到83.2%。酶分配于顶相,水解产物分配于底相,混合氨基酸的总收率为76.7%。水解产物中混合氨基酸由13种氨基酸组成,其中7种为必须氨基酸。

关键词: 双水相系统, 明胶, 水解, 氨基酸

Abstract: s:To produce amino-acids through gelatin hydrolysis, catalyzed by protease by aqueous two -phase systems was studied. The constituted 20% PEG1540 with 10% potassium phosphate by the aqueous two phase system with 3% alkali protease and 35% gelatin added was hydrolyzed at the conditions of pH7.5 and 45℃ for 10h. With the hydrolysis of 83.2%, 76. 7% amino acids was obtained, totally constituted 13 kinds of amino acids.

Key words: two-- phase system, gelatin, hydrolysis, amino acids