低温纤维素酶产生菌的筛选、鉴定及酶学性质初步研究

食品科学 ›› 2007, Vol. 28 ›› Issue (12): 235-239.

低温纤维素酶产生菌的筛选、鉴定及酶学性质初步研究

吕明生, 吕凤霞, 房耀维, 刘殊, 王淑军

淮海工学院江苏省海洋生物技术重点建设实验室；南京农业大学食品科技学院；淮海工学院江苏省海洋生物技术重点建设实验室江苏连云港222005；江苏南京210095；江苏连云港222005；江苏连云港222005南京农业大学食品科技学院；

出版日期:2007-12-15 发布日期:2011-11-22

Screening and Identification of A Cold-adapted Cellulase-producing Strain and Characterization of Cellulase

吕Ming-Sheng , 吕Feng-Xia , FANG Yao-Wei, LIU Shu, WANG Shu-Jun

1.Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China; 2.College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Online:2007-12-15 Published:2011-11-22

摘要/Abstract

摘要： 从连云港高公岛海域采集海水和海泥样品,通过2216E平板初筛和摇瓶发酵复筛,得到一株产纤维素酶的菌株Z6。对该菌生物学特性进行研究,菌株Z6为革兰氏阴性杆状细菌、有荚膜、无芽孢。菌的生长温度范围为4～35℃,最适生长温度为25℃;最适生长pH为8;最适NaCl浓度为3%。根据其形态特征、生理生化特性和16SrRNA序列分析结果,初步鉴定为交替假单孢菌属(Pseudoalteromonassp.)。根据菌株16SrRNA同源性和系统进化树分析进一步鉴定菌株Z6属于Pseudoalteromonas carrageenovora。目前尚无Pseudoalteromonas carrageenovora产生低温纤维素酶的报道。菌株Z6所产纤维素酶的最适作用温度和pH分别为30℃和pH8,在10℃仍具有较高活性,具有较大的潜在工业应用价值。

关键词: 低温纤维素酶, 交替假单孢菌, 筛选, 鉴定, 酶学性质

Abstract: Strain Z6 producing extracellular cellulase was isolated from seawater and sea mud collected from the Gaogong island of Lianyungang seaside. Its cell was rod shaped and gram-negative, with capsule and non-sporeforming. The fit range of growth temperature was 4～35 ℃, and the most suitable one was 25 ℃. The optimum pH for its growth was at pH8. The most suitable concentration of NaCl was 3%. The preliminary identification indicated that the strain belonged to Pseudoalteromonas sp. according to its morphological, the physiological and biochemical properties and 16SrRNA sequence analysis. Sequencing and phylogenetic analysis of the 16S rRNA gene of strain Z6 indicated that the strain belonged to Pseudoalteromonas carrageenovora. No work on Pseudoalteromonas carrageenovora producing cold-adapted cellulase had been reported so far. The optimal temperature and optimal pH for the cellulase produced by strain Z6 were 30℃ and 8.0 respectively. The cellulase still kept high activity at 10 ℃. It might have a potential value of industrial application.

Key words: cold-adapted cellulase, Pseudoalteromonas carrageenovora, screening, identification, enzyme charac-terization

吕明生, 吕凤霞, 房耀维, 刘殊, 王淑军. 低温纤维素酶产生菌的筛选、鉴定及酶学性质初步研究[J]. 食品科学, 2007, 28(12): 235-239.

吕Ming-Sheng , 吕Feng-Xia , FANG Yao-Wei, LIU Shu, WANG Shu-Jun. Screening and Identification of A Cold-adapted Cellulase-producing Strain and Characterization of Cellulase[J]. FOOD SCIENCE, 2007, 28(12): 235-239.

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