食品科学 ›› 2004, Vol. 25 ›› Issue (1): 163-166.

• 营养卫生 • 上一篇    下一篇

白蛋白多肽对核苷的促吸收及增强小鼠细胞免疫功能的作用

 陈栋梁, 刘莉, 于兰, 殷腊生, 王阿敬, 杨渝珍   

  1. 武汉肽类物质应用研究中心,华中科技大学同济医学院实验医学研究中心
  • 出版日期:2004-01-15 发布日期:2011-10-24

The Promoting Effect of Albumin Polypeptide on Absorption of Nucleosideand Cellular Immune Function in Mice

 CHEN  Dong-Liang, LIU  Li, YU  Lan, YIN  La-Sheng, WANG  A-Jing, YANG  Yu-Zhen   

  1. 1.Wuhan Application Research Center for Peptide Substance;2.Research Center of Experi-mental Medicine, Tongji Medical Cellage, Huazhong Science and Technology University
  • Online:2004-01-15 Published:2011-10-24

摘要: 目的:观察白蛋白多肽促进核苷在肠道的吸收及对细胞免疫功能的增强作用。方法:应用同位素示踪法对小鼠小肠、肝及胸腺等组织的3H-胸腺嘧啶核苷(3H-TdR)放射性值进行检测,比较了白蛋白多肽和3H-TdR混合物进食组小鼠与仅进食3H-TdR组小鼠间的区别。此外,应用MTT法对实验鼠ConA刺激的脾淋巴细胞增殖活性进行了测定。结果与结论:白蛋白多肽具有促进核苷在小肠的吸收及转运作用并增加在肝和胸腺组织的分布。此外,白蛋白多肽和核酸混合灌胃组小鼠的脾淋巴细胞增殖活性高于单独白蛋白多肽或单独核酸灌胃组小鼠。结果表明,白蛋白多肽具有促进核苷的吸收及利用作用,并对动物机体细胞免疫功能具有增强作用。

关键词: 细胞免疫, 3H-胸腺嘧啶核苷, 白蛋白多肽

Abstract: Purpose:To observe the promoting effect of albumin polypeptide on absorption of nucleoside in intestinal andon cellular immune function in tested mice. Method:The radioactive values of 3H-TdR in intestinal,liver, thymus etc. weredetected using isotope trace method. The group of mixture dieted albumin polypeptide with 3H-TdR was compared with the groupdieted only 3H-TdR in mice. At the same time, the proliferation activity of splenic lymphocytes stimulated by Con A was alsodetected using MTT method. Results and Conclusion:The albumin polypeptide is able to promote the absorption andtransport of nucleoside in intestinal and the distribution of 3H-TdR into liver and thymus. Furthermore, the proliferation activityof splenic lymphocytes from the test groups in which the mixture diet of albumin polypeptide and nucleic acid was poured tostomach of mice was higher then control group. The results show that the both of dietary albumin polypeptides and nucleic acidas a new type of conditional nutrition source can increase the cellular immune function of animal body.

Key words: cellular immunity, 3H-TdR, albumin polypeptide