食品科学

• 营养卫生 • 上一篇    下一篇

中国蛤蜊精对小鼠免疫活性观察

史 倩1,王晓洁1,*,张志伟1,刘晓晨1,梁建光1,王守海1,刘新生2,曲彦清2   

  1. 1.鲁东大学生命科学学院,山东 烟台 264025;2.鲁东大学校医院,山东 烟台 264025
  • 出版日期:2013-09-15 发布日期:2013-09-27
  • 通讯作者: 王晓洁
  • 基金资助:

    山东省科技厅星火计划项目(2011SH05012)

Immunoenhancing Activity of Mactra chinensis Meat Extract in Mice

SHI Qian1,WANG Xiao-jie1,*,ZHANG Zhi-wei1,LIU Xiao-chen1,LIANG Jian-guang1,WANG Shou-hai1,LIU Xin-sheng2,QU Yan-qing2   

  1. 1. College of Life Science, Ludong University, Yantai 264025, China;
    2. College of Hospital, Ludong University, Yantai 264025, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: WANG Xiao-jie

摘要:

目的:观察中国蛤蜊精(每100g中,含多糖19.01g、牛磺酸1.25g、胆碱0.65g)对小鼠免疫功能的影响。方法:用中国蛤蜊精按0.5、1.5、3.0g/(kg·d)的剂量对小鼠灌胃,连续10d,测定小鼠白细胞总数及分类计数、通过抗体凝集反应实验测定效价、测定抗体生成细胞水平、腹腔巨噬细胞吞噬功能、体质量变化及免疫器官质量,并对其结果进行统计分析。结果:与生理盐水组(对照组)相比,中国蛤蜊精各剂量组淋巴细胞相对数、抗体生成水平、抗体生成细胞水平、小鼠腹腔巨噬细胞吞噬功能和脾脏指数均显著升高(P<0.05)。结论:中国蛤蜊精有显著增强小鼠免疫功能的作用。

关键词: 中国蛤蜊精, 细胞免疫, 体液免疫, 非特异性免疫

Abstract:

Objective: To observe the immunoregulatory effect of Mactra chinensis meat extract (EMC, containing 19.01 g
of polysaccharide, 1.25 g of taurine and 0.65 g of choline /100 g) in mice. Methods: Mice were orally administered with EMC
at doses of 0.5, 1.5, 3.0 g/(kg•d) for 10 d. A series of immune function indices such as the total number and classification of
white blood cells (WBC), the titer of antibody production in agglutination reaction and the quantity of plaque-forming cells
(PFC), macrophage phagocytosis test, and the weight of body and immune organs were analyzed. Results: Compared with
the control group, the relative number of lymphocytes, the activity of antibody production, the quantity of antibody-forming
cells, the phagocytic function of mouse peritoneal macrophages and the index of spleen in all the treated groups were improved
significantly (P <0.05). Conclusion: EMC enhances the immune activity of normal mice effectively.

Key words: water extract from Mactra chinensis (EMC), cellular immunity, humoral immunity, nonspecific immunity

中图分类号: